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vaccinia

Examples

  • Progressive vaccinia (vaccinia necrosum, vaccinia gangrenosa) is a severe, potentially fatal illness characterised by progressive necrosis at the vaccination site, often leading to metastatic lesions. It was observed almost exclusively among immunodeficient persons. — “Vaccinia (Progressive vaccinia/vaccinia necrosum) & Eczema”, whale.to
  • Vaccinia definition, a variant of the cowpox virus that became established in vaccines derived from cowpox-inoculated humans. See more. — “Vaccinia | Define Vaccinia at ”,
  • vaccinia ( vak′sinēə ) ( veterinary medicine ) A contagious disease of cows which is characterized by vesicopustular lesions of the skin that are. — “Vaccinia: Definition from ”,
  • laboratory and health-care workers occupationally exposed to vaccinia virus, In addition, this report contains ACIP's recommendations for the use of vaccinia. — “Vaccinia (Smallpox) Vaccine”, hhs.gov
  • Definition of vaccinia in the Medical Dictionary. vaccinia explanation. Information about vaccinia in Free online English dictionary. What is vaccinia? Meaning of vaccinia medical term. What does vaccinia mean?. — “vaccinia - definition of vaccinia in the Medical dictionary”, medical-
  • A schematic 3D model of the vaccinia virion. We present in this web The model was constructed to accompany a review of vaccinia virus structure and assembly (Condit et al., 2006). — “Vaccinia Viron - 3D Tour”,
  • Vaccinia symptoms, causes, diagnosis, and treatment information for Vaccinia (Vaccinia) with alternative diagnoses, full-text book chapters, misdiagnosis, research treatments, prevention, and prognosis. — “Vaccinia - ”,
  • Translations of vaccinia. vaccinia synonyms, vaccinia antonyms. Information about vaccinia in the free online English dictionary and encyclopedia. vaccinia - a local infection induced in humans by inoculation with the virus causing cowpox in order to confer resistance to smallpox; normally lasts. — “vaccinia - definition of vaccinia by the Free Online”,
  • Vaccinia is so mild that it is typically asymptomatic in healthy individuals, but may causes a mild rash and The body's immune response to vaccinia also immunizes against smallpox, and for this reason, vaccinia is used as a live-virus innoculator against smallpox. — “Vaccinia - Wikinfo”,
  • The dimensions of the virion are roughly 360 × 270 × 250 nm, with a mass of approximately 5-10 fg.[2] Vaccinia virus is well-known for its role as a vaccine that eradicated the smallpox disease, making it the first human disease to be successfully eradicated by science. — “Vaccinia - Wikipedia, the free encyclopedia”,
  • Overview: Vaccination with vaccinia virus has been directly responsible for the successful eradication of smallpox (variola). Although the exact origins of vaccinia virus are uncertain, vaccinia may represent a hybrid of the variola and cowpox. — “Vaccinia: eMedicine Infectious Diseases”,
  • Vaccinia vaccine does not contain smallpox (variola) virus. Previously, the vaccine had been prepared from calf lymph with a seed virus derived from the New York City Board of Health (NYCBOH) strain of vaccinia virus and has a minimum concentration of 108 pock-forming units (PFU)/ml. — “Vaccinia (Smallpox) Vaccine”, cdc.gov
  • Vaccinia virus, a virus related to cowpox virus, is a double-stranded The consequences of transfusion-transmitted vaccinia virus could include severe complications of vaccinia infection. — “Guidance for Industry: Recommendations for Deferral of Donors”, fda.gov
  • Several cases of laboratory-acquired Vaccinia virus infection associated with genetic modification work are known to have occurred in the UK in recent years. Sharps use should be avoided as inoculation injuries carry a much higher risk of transmission of infection. — “Vaccinia, University Occupational Health Service”,
  • Vaccinia Virus Use & Immunization Policy for Laboratory Personnel Recombinant vaccinia and other pox viruses are useful microbiological research tools for expression of exogenous proteins in a variety of. — “Vaccinia Virus, U.Va”, ehs.virginia.edu
  • Vaccinia virus is a big mystery in virology. It is not known whether vaccinia virus is the product of genetic recombination, or if it is a species derived from cowpox virus or variola virus by prolonged serial passage, or if it is the living representative of a now extinct virus. — “VACCINIA VIRUS”, stanford.edu
  • Vaccinia virus can be transmitted from the vaccination site to close contacts of persons Unintentional transfer of vaccinia virus includes transfer from the vaccination site to. — “Surveillance guidelines for smallpox vaccine (vaccinia”, guideline.gov
  • Material Safety Data Sheets (MSDS), regulated under Workplace Hazardous Materials Information System (WHMIS) legislation, for chemical products have been available to workers for many years. However because many laboratory workers, whether in. — “Vaccinia virus - Material Safety Data Sheets (MSDS)”, phac-aspc.gc.ca
  • Vaccinia virus (VACV or VV) is a large, complex, enveloped virus belonging to the poxvirus family. Vaccinia virus is well-known for its role as a vaccine that eradicated the smallpox disease, making it the first human disease to be successfully eradicated by science. — “Vaccinia”,

Videos

  • The Benefits of Open Access and Publishing with BioMed Central Raymond Weinstein is one of our authors and in this video he talks about the benefits of open access and publishing with BioMed Central. You can access Raymond's article in BMC Immunology, entitled 'Significantly reduced CCR5-tropic HIV-1 replication in vitro in cells from subjects previously immunized with Vaccinia Virus' here:
  • Would it be OK for Lindsay Lohan to sleep with Tony Allen? Lohan sleeping with a married man. Who's fault is it, her's or the married guys? Watch the entire show at A British heiress has started divorce proceedings in the wake of claims that Lindsay Lohan had *** with her musician husband in the toilet of a rehab clinic. Sensational divorce papers identifying Mean Girls star Lohan were filed last week by US lawyers acting for Stephanie Allen, 28, whose family is worth £720 million. Mother-of-two Stephanie, from Lincolnshire, pulls no punches against her husband Tony, 39, the Dead Stays Alive frontman, in the 52-pages filed in Chatham County Court in Savannah, Georgia. Stephanie states that her three-year marriage "is irretrievably broken" and gives the grounds for divorce as his "adultery" and "cruel treatment". The papers also accuse her husband of being "guilty of habitual drug addiction" and that she should retain custody of their eight-month-old twins. The lawyers do not name Lohan in the actual complaint but they refer to her husband's "conduct and relationship with another woman" as described in an American magazine and on showbiz internet sites. But Exhibit 'A', which is attached to the divorce papers, includes articles claiming Lohan, 21, who has had an on-off affair with Calum Best, started a relationship with Tony Allen while they were both being treated at the Cirque Lodge clinic in Utah. One of the articles attached to the exhibit was featured last week in America's In Touch magazine. It states ...
  • Wellcome Trust Annual Review 2008 (HD) | A film by the Wellcome Trust In this video, Sir Mark Walport (Director of the Wellcome Trust) and others review some of the Trust's activities during 2008, with a particular focus on our partnerships. These have included Project Enthuse - funding science training for teachers, the search for a new TB vaccine, our involvement with the debate surrounding the Human Fertilisation and Embryology Bill and the continuing activites of Wellcome Collection. For more information and the print version of the annual review go to
  • Virus plaque formation Movie showing the spread and plaque formation of VACV Virus expressiong GFP protein extracted from supporting material of the following article: Repulsion of Superinfecting Virions: A Mechanism for Rapid Virus Spread Virginie Doceul, Michael Hollinshead, Lonneke van der Linden, and Geoffrey L. Smith (21 January 2010) Science [DOI: 10.1126/science.1183173] For more, please look here:
  • vaccinia virus moving inside an infected cell Widefield movie of GFP-tagged vaccinia virus particles (green dots) moving on tips of actin tails (Red) in infected cell expressing RFP-tagged actin. Green virus particles also move indpendently of actin tails in the cytoplasm of the infected cell using microtubules.
  • Smallpox Vaccine 1955 The smallpox vaccine helps the body develop immunity to smallpox. The vaccine is made from a virus called vaccinia which is a pox-type virus related to smallpox. The smallpox vaccine contains the live vaccinia virus—not dead virus like many other vaccines. For that reason, the vaccination site must be cared for carefully to prevent the virus from spreading. Also, the vaccine can have side effects. In the past, about 1000 people for every 1 million people vaccinated for the first time experienced reactions that, while not life-threatening, were serious. These reactions included a toxic or allergic reaction at the site of the vaccination (erythema multiforme), spread of the vaccinia virus to other parts of the body and to other individuals (inadvertent inoculation), and spread of the vaccinia virus to other parts of the body through the blood (generalized vaccinia). These types of reactions may require medical attention. In the past, between 14 and 52 people out of every 1 million people vaccinated for the first time experienced potentially life-threatening reactions to the vaccine. Based on past experience, it is estimated that 1 or 2 people in 1 million who receive the vaccine may die as a result. Routine smallpox vaccinations were discontinued among US children in 1972, and among US healthcare workers in 1976 after the disease was eradicated in the United States and because of the side effects. Until recently, the US government provided the vaccine only to a few hundred ...
  • vaccinia virus induced actin tails Confocal movie of a cell expressing GFP-Actin reveals that infection with vaccinia virus for 8 hours results in the formation of actin tails that move the virus at 0.2 um/sec.
  • AIDS laced in medicine Bayer is a global enterprise with core competencies in the fields of health care, nutrition and high-tech materials. The Real Origin of AIDS The AIDS virus was created by the United States government at Fort Dietrich in Maryland, a biological warfare laboratory in building number A550 in the P4 lab. The sum of US $10m was requested by the Defense Department to build the lab under the House of Representatives Bill number HR 15090 in the 91st Congress in 1970.7 Dr. Theodore Strecker writes, "The US National Cancer institute, in collaboration with the World Health Organization, manufactured the AIDS virus in their laboratories at Fort Detrich, Maryland. They combined two deadly retroviruses, the Bovine Leukemia Virus and the Visna Virus, and injected them into human tissue cultures. The result was the AIDS virus, the first human retrovirus known to man and now believed to be 100 percent fatal to those infected".8 Dr. Strecker further contends that AIDS couldn't have engineered itself, and reiterates that it was produced in a laboratory by virologists. "The World Health Organization (WHO) called for scientists to work with these deadly agents and attempt to make a hybrid virus that would be deadly to humans. An attempt was made to see if viruses could in fact exert selective effects on immune function. The possibility was looked into that the immune response to the virus itself would be impaired if the infecting virus damaged, more or less selectively, the cell responding to ...
  • Smallpox This patient education video explains what smallpox is and discusses the benefits and risks of vaccination. People who should not be vaccinated are discussed. Self-care instructions are included for people who have received the smallpox vaccine.
  • Vaccinia virus moving on microtubules in an infected cell Spinning disc confocal movie of GFP tagged vaccinia virus moving to cell periphery on GFP-tagged microtubules 6 hours post infection.
  • Vaccinia virus induced cell migration Phase images reval that Vaccinia virus infection induces rapid motility of BSC 1 cells in a scratch assay in the absence of serum over a period of 15 hours. Compare to our other video of non-infected cells
  • Ari Helenius (ETH Zurich) Part 2 Endocytosis and Penetration In the second lecture, the next steps in viral infection are described. Endocytosis of plasma membrane bound viruses can occur via a number of mechanisms including caveolar, clathrin, non-clathrin, or lipid raft mediated pathways. The internalized virus is enclosed in an endosome that may undergo increasing acidification resulting in acid mediated fusion between the viral envelope and the vesicle membrane. Following membrane penetration, the virus, once again, makes use of cellular machinery such as microtubules and their motors, to transfer its genome to the nucleus. Helenius describes experiments from his lab and others that have deciphered these complex processes. See more at
  • Rich Condit reminisces On my recent trip to record TWiV #111 at Florida Gulf Coast University, I visited Rich Condit in Gainesville. There he told me a story about how the bacteriophage T7 polymerase/promoter system was developed. It's an interesting tale that demonstrates how important scientific advances often have convoluted roots.
  • The Truth About Vaccines Part 1 Vaccines are packed with toxic substances that destroy the immune system and overall health of the body. This following list of common vaccines and their ingredients should shock anyone. The numbers of microbes, antibiotics, chemicals, heavy metals and animal byproducts is staggering. DPT and DTaP - Diphtheria-Tet***-Pertussis diphtheria and tet*** toxoids and acellular pertussis adsorbed, formaldehyde, aluminum phosphate, ammonium sulfate, and thimerosal, washed sheep RBCs (aluminum hydroxide in the DTaP shots) Influenza B Haemophilus influenza Type B, polyribosylribitol phosphate ammonium sulfate HiB Titer - Haemophilus Influenza B haemophilus influenza B, polyribosylribitol phosphate, yeast, ammonium sulfate, thimerosal, and chemically defined yeast-based medium IPOL 3 types of polio viruses neomycin, streptomycin, and polymyxin B formaldehyde, and 2-phenoxyethenol continuous line of monkey kidney cells Attenuvax - Measles measles live virus neomycin sorbitol hydrolized gelatin, chick embryo Biavax - Rubella rubella live virus neomycin sorbitol hydrolized gelatin, human diploid cells from aborted fetal tissue BioThrax - Anthrax nonencapsulated strain of Bacillus anthracis aluminum hydroxide, benzethonium chloride, and formaldehyde Dryvax - Smallpox Live vaccinia virus, with "some microbial contaminants," polymyxcin B sulfate, streptomycin sulfate, chlortetracycline hydrochloride, and neomycin sulfate glycerin, and phenol -a compound obtained by distillation of coal ...
  • New Smallpox Vaccine Licensed FDA recently licensed a new smallpox vaccine called ACAM2000 which is made by Acambis Inc. This new vaccine is not available for routine use, but will be stored in the country's Strategic National Stockpile. It could be used to inoculate people at high risk of smallpox exposure during a bioterrorist attack. ACAM2000 is derived from the Dryvax smallpox vaccine, which is no longer made and in limited supply. The new vaccine is made with modern cell culture technology, which allows the vaccine to be produced on a large scale with more consistent quality. The new vaccine was studied in two populations: those who'd never been vaccinated for smallpox and those who'd been vaccinated many years before. The percentage of unvaccinated people who had a successful immunization reaction was similar to that of the older vaccine. The new vaccine was also found to be acceptable as a booster for those previously vaccinated for smallpox. Because the new vaccine contains live vaccinia virus, vaccinated people must be careful not to spread the virus from the inoculation site to other parts of their body or to other people. People who receive the vaccine must be given a Medication Guide that explains how to care for the vaccination site properly and lists the serious side effects that can occur. FDA Patient Safety News: November 2007 For more information, please see our website: www.accessdata.fda.gov
  • Vesicles on the Move Endosomal vesicles rocketing through Xenopus laevis cytoplasm, powered by dendritic actin network assembly and disassembly.
  • Engineered Biologicals Report 8/24/09 It appears that some new forms of new organisms being manufactured right now. 1. Spliced HIV-4 virus, human tissue splice from Spleen. 2. Flavivirus (similar to Ebola) 3. Hepatitis type G, engineered, new strain. 4. Rhabidoviridae engineered- Rabies, spliced with human tissue from kidneys, will attack kidneys. 5. Vaccinia virus engineered- Smallpox 6. Polonium- radioactive element always present in the cancer causing chain of chemicals and biologicals. 7. Cancer cells, specifically attack uterine tissue. Where do they plan to use this mix? West Coast, USA When? 8 weeks- November, 2009 How? 1. aerosol, maybe chemtrails, 2. bottled water, coca-cola figures strongly in this operation (Dasani, Aquarius, Glaceau). Not mentioned- Pepsi has Gatorade, Aquafina, Sobe, Ethos. Nestle has Arrowhead, Zephyr Hills, Pure Life, Deer Park, Calistoga, Ice Mountain, Ozarka, Poland Springs, etc. This report has not been published. This is one of very few places this information is available. How one chooses to use this information is purely personal. This information is made available without warranty but it should be noted that the resonance testers who provided the results have accuracy levels above 90%. Depopulation. Information on this subject is coming out everywhere, with sources and reasons almost unlimited. Just search it out. Bottled water- demographics show mostly young people drink bottled water. The vaccine is also said to target this same group. No one would ever trace the ...
  • MTR uptake in MDCK cells Images in this movie were taken on a confocal microscope.
  • Ari Helenius (ETH Zurich) Part 3 Open Sesame: Cell Entry and Vaccinia Virus Part 3 focuses on a single virus, the Vaccinia virus, as a model for cell binding, signaling and endocytosis. Fluorescently labeled Vaccinia viruses bind to and surf along host cell filopodia. Helenius lab members noticed that when Vaccinia, unlike other viruses, reached the surface of the cell body it caused the plasma membrane to form blebs. Further experiments showed that the virus tricks the cell into thinking it is apoptotic debris. This induces blebbing and subsequent uptake of the virus by macropinocytosis. Additionally, automated high throughput siRNA screening was used to screen a large number of infected cells for host genes required for Vaccinia virus uptake. ***ysis of the genes identified allowed host factors and processes critical to viral infection to be identified. Expansion of this technique may provide a new source of information on pathogen-host interactions. See more at
  • The Truth About Vaccines Part 2 Vaccines are packed with toxic substances that destroy the immune system and overall health of the body. This following list of common vaccines and their ingredients should shock anyone. The numbers of microbes, antibiotics, chemicals, heavy metals and animal byproducts is staggering. DPT and DTaP - Diphtheria-Tet***-Pertussis diphtheria and tet*** toxoids and acellular pertussis adsorbed, formaldehyde, aluminum phosphate, ammonium sulfate, and thimerosal, washed sheep RBCs (aluminum hydroxide in the DTaP shots) Influenza B Haemophilus influenza Type B, polyribosylribitol phosphate ammonium sulfate HiB Titer - Haemophilus Influenza B haemophilus influenza B, polyribosylribitol phosphate, yeast, ammonium sulfate, thimerosal, and chemically defined yeast-based medium IPOL 3 types of polio viruses neomycin, streptomycin, and polymyxin B formaldehyde, and 2-phenoxyethenol continuous line of monkey kidney cells Attenuvax - Measles measles live virus neomycin sorbitol hydrolized gelatin, chick embryo Biavax - Rubella rubella live virus neomycin sorbitol hydrolized gelatin, human diploid cells from aborted fetal tissue BioThrax - Anthrax nonencapsulated strain of Bacillus anthracis aluminum hydroxide, benzethonium chloride, and formaldehyde Dryvax - Smallpox Live vaccinia virus, with "some microbial contaminants," polymyxcin B sulfate, streptomycin sulfate, chlortetracycline hydrochloride, and neomycin sulfate glycerin, and phenol -a compound obtained by distillation of coal ...
  • Actin based motility of vaccinia virus Widefield movie of GFP-tagged vaccinia virus particles (green dots) moving on tips of actin tails (Red) in infected cell expressing RFP-tagged actin. Green virus particles also move indpendently of actin tails in the cytoplasm of the infected cell using microtubules
  • MDiTV News: Virus Spreads Faster than Previously Thought Imagine a virus surfing from cell to cell in your body rapidly infecting you at a rate scientists never thought possible. The researchers at Imperial College London were able to capture a virus on film that spreads like wildfire. It's the vaccinia vaccinia virus, the one used to eradicate smallpox, and it is challenging the way scientists understood how viruses spread. Researchers were shocked by how fast the virus spread through cells in a lab dish, and these guys know the vaccinia virus as intimately as anyone (videos, BBC News). This kind of surprise sounds as though it would be bad news, but the video may offer some useful clues for fighting viral infections. Previously viruses were believed to spread through entering a cell and replicating inside before moving on to other cells. So the virus could only spread depending on how fast it could replicate.
  • HPV-16 Moving on a Cell "HPV-16 motion on actin-rich protrusions of HeLa cells. Movie from an epifluorescence microscopy time series showing several AF488 labeled HPV-16 particles moving along actin-rich protrusions on HeLa cells expressing EGFP-actin. Acquisition of images occured at 2 frames per s, movie played at 10 Hz." Source: Human Papillomavirus Type 16 Entry: Retrograde Cell Surface Transport along Actin-Rich Protrusions Authors: Mario Schelhaas, Helge Ewers, Minna-Liisa Rajamäki, Patricia M. Day, John T. Schiller, and Ari Helenius PLoS Pathog. 2008 September; 4(9): e1000148. Published online 2008 September 5. doi: 10.1371/journal.ppat.1000148. Copyright This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
  • GeoVax, Inc - AIDS Vaccine Research, Vaccine Awareness GOVX Geovax Labs, Inc. (GOVX.OB), Vaccine Technology GeoVax Labs, Inc., a clinical stage biotechnology company, develops human vaccines for diseases caused by human immunodeficiency virus (HIV) and other infectious agents. Its DNA (deoxyribonucleic acid) and MVA (Modified Vaccinia Ankara) vaccines are in Phase II human clinical trial by the HIV Vaccine Trials Network. The company was founded in 2001 and is based in Atlanta, Georgia. GeoVax's AIDS (HIV-1) Vaccines, initially developed at Emory University by Dr. Harriet Robinson, currently GeoVax's Senior Vice President of Research and Development, in collaboration with researchers at the NIAID (NIH) and the US Centers for Disease Control (CDC), are recombinant DNA and MVA vaccines. Our focus is on developing AIDS vaccines comprising the major HIV-1 subtypes (A, B and C). These vaccines can be used alone or in combination depending on a local infection. Our first priority is subtype B which is most common in North America, the European Union, Japan and Australia. When administered in series, our AIDS vaccines induce strong cellular and humoral immunity (protection) in non human primates against multiple HIV-1 (AIDS virus) proteins. This suggests that GeoVax's vaccines will provide protection against the development of AIDS in HIV-1 virus infected people. Our Progress * Excellent Preclinical Data * Phase 1 Preventative Human Trial of a Prototype DNA Completed Satisfactory * Four Phase 1A/IB Preventative Human Trials Combining DNA ...
  • RNAi Screening for Host Factors Involved in Vaccinia Virus Infection using Drosophila Cells Watch video protocols on JoVE - . Journal of Visualized Experiments (JoVE) is a scientific journal publishing video articles on biological experiments filmed at leading research institutions. JoVE is the first video journal indexed in PubMed. To see more of this protocol go to RNAi Screening for Host Factors Involved in Vaccinia Virus Infection using Drosophila Cells This video protocol was filmed at the University of Pennsylvania. Viral pathogens represent a significant public health threat; not only can viruses cause natural epidemics of human disease, but their potential use in bioterrorism is also a concern. A better understanding of the cellular factors that impact infection would facilitate the development of much-needed therapeutics. Recent advances in RNA interference (RNAi) technology coupled with complete genome sequencing of several organisms has led to the optimization of genome-wide, cell-based loss-of-function screens. Drosophila cells are particularly amenable to genome-scale screens because of the ease and efficiency of RNAi in this system 1. Importantly, a wide variety of viruses can infect Drosophila cells, including a number of mammalian viruses of medical and agricultural importance 2,3,4. Previous RNAi screens in Drosophila have identified host factors that are required for various steps in virus infection including entry, translation and RNA replication 5. Moreover, many of the cellular factors required for viral replication ...
  • actin motility actin motility assay done in Woods Hole 2009