Chatter suggests that granules break upon sectioning c=cornea PC=pseudocone p=primary pigment cell s=secondary pigment cell R=rhabdomere SC Semper cell From Stark et al 1988 Using optical neutralization of the cornea pigmented primary pigment cells secondary pigment cells and receptor cells can be individually identified in eye color pigment mosaics A giant
A low voltage scanning electron micrograph LVSEM of the pseudocone and surrounding cells and rhabdomere tips top and of rhabdomeric microvilli bottom see Juang et al Electron micrographs from Stark and Carlson 1982 showing degeneration in rdgB after one week in cyclic light of R1 6 but sparing or R8 and post synaptic cells This page was last updated
Stark W S The effects of temperature on visual receptors in temperature sensitive paralytic paralytic shibire ***s mutant Drosophila Journal of Insect Physiology 1993 39 385 392 Coated pit suggesting autophagy from retinula cell plasmalemma Also note rhabdomere tip see Stark et al 1988 Coated pit suggesting autophagy from rhabdomere see Stark et al 1988
is decreased but not eliminated by vitamin A deprivation Similarly Rh4 s promoter 373 +83 drives fluorescence in R7 in this case in the rhabdomere and the cell body Again vitamin A deprivation decreases but does not eliminate this fluorescence Earlier we had shown that a yeast glucose food that lacks chromophore precursors and hence rhodopsin does have
iv Sodium calcium exchange Living dissociated Drosophila ommatidia The light absorbing rhabdomere has been labelled by a genetically targeted GFP tagged ion channel protein Kir2 1 As well as visualizing the
protein namely opsin In retinoid deprived Drosophila sensitivity visual pigment P face particles rhodopsin immunogold labeling and rhabdomere size are greatly reduced Here is a Transmission electron micrograph showing that rhabdomeres of retinoid deprived Drosophila are smaller than those of replete flies from Sapp et al 1991 Recovery is rapid when deprived
Pineda is helping me to put the finishing touches on this work More recently Dr Pichaud provided flies with the R7 opsin promoters driving GFP directly Rh3 s promoter 345 +18 drives fluorescence in R7 s rhabdomere This fluorescence is decreased but not eliminated by vitamin A deprivation Similarly Rh4 s promoter 373 +83 drives fluorescence in R7 in this case in
Rhabdomere Here are cross sectional images of the rhabdomere array Although the cell structure is heavily damaged and very brittle it was posible to break the strucutes down the middle exposing
touches on this work More recently Dr Pichaud provided flies with the R7 opsin promoters driving GFP directly Rh3 s promoter 345 +18 drives fluorescence in R7 s rhabdomere This fluorescence is decreased but not eliminated by vitamin A deprivation Similarly Rh4 s promoter 373 +83 drives fluorescence in R7 in this case in the rhabdomere and the cell body Again
Rhabdomere Here are cross sectional images of the rhabdomere array Although the cell structure is heavily damaged and very brittle it was posible to break the strucutes down the middle exposing
Rh3 s promoter 345 +18 drives fluorescence in R7 s rhabdomere This fluorescence is decreased but not eliminated by vitamin A deprivation Similarly Rh4 s promoter 373 +83 drives fluorescence in R7 in this case in the rhabdomere and the cell body Again vitamin A deprivation decreases but does not eliminate this fluorescence Earlier we had shown that a yeast
Click on image to view larger version Figure 5 WASp is required for rhabdomere morphogenesis A Phase contrast image of 2 µm section of WASp3 adult eye mosaic clones Mutant tissue can be identified by the lack of red
Two types of eyes either 1 cilia or 2 rhabdomere source
a yeast glucose food that lacks chromophore precursors and hence rhodopsin does have substances that activate transcription This is dramatized in Rh4 promoter GFP reporter flies by the high fluorescence of R7 It is interesting to note that the difference in appearance of the deep pseudopupil for Rh3 vs Rh4 rhabdomere only vs rhabdomere plus cell body was also observed in
blocked and the rhabdomeres become separated creating the inter rhabdomal space in which the microvilli will be located In insects with apposition eyes the rhabdomeres remained fused Zelhof and his colleagues screened different lines of chemically mutagenized fruit flies using electron microscopy to look for specimens with altered rhabdomere topology Two of these lines
