3D Reconstruction of Liver Portal Tracts From a Normal and Abnormal Dog See the anaglyph version (you will need red-green/blue glasses) at This video compares the hepatic portal track of a normal dog's liver to a dog with a congenital vascular abnormality termed a portosystemic shunt. In this condition the portal vein has an abnormal connection to the systemic venous system and deprives the liver of the normal portal blood flow. Red=hepatic arteries, Blue=portal vein, Green=bile duct, Yellow=lymphatic space. In the normal portal tract the size and relationship of the portal vein, the hepatic artery, bile ducts and the lymphatic spaces can be seen. The hepatic portal tract from the dog with the portosystemic shunt is characterized by the lack of a portal vein, dramatically distended lymphatic space and an increased size with multiple small branches of the hepatic artery. This negative-space filling technique was performed on a small number of sections (normal dog=40, shunt dog=48) from FAA-fixed, paraffin-embedded liver tissue. Paraffin blocks were sectioned at 10 microns and treated with a standard H&E stain. Sections were photographed with a Sony DSC707 consumer-grade camera on a Nikon Eclipse 50i at 200X. JPEG images were imported into Adobe After Effects on a Macintosh desk-top computer. Details of the process used to build the 3D volumes are described in a recently published article: www.ars.usda.gov and in our other video: and The 3D images are not cartoons but represent the actual empty space inside the ...
Stratatech's StrataTest® Full-Thickness Human Skin Model Composed of both an epidermis and a dermis, the StrataTest® full-thickness human skin model displays the physical, chemical and histological of native human skin. The tissue is readily available, reliable and is supplied in an easy-to-use, 24-well format for consumer product testing, drug discovery and toxicity screening.
3D Reconstruction of a Canine Liver Portal Tract II 199 Trichome-stained sections of a portal tract from a normal dog were aligned and the negative-space in each image was colorized as follows: Blue: Portal vein branches Red: Arteries Green : Bile ducts Yellow: Lymphatic channels Images were then stacked, producing a 3D reconstruction of the vessels within the tract. Details: 0.6 micron sections were photographed with a Sony DSC707 consumer-grade camera on a Nikon Eclipse 50i at 100X. JPEG images were imported into Adobe After Effects on a Macintosh desk-top computer. More detail of the process used to build the 3D volume is described in a recently published article: www.ars.usda.gov and in our other video: The 3D reconstructions are not cartoons but represent the actual empty space inside the vessels in each image. The edge of each vessel was determined using Roto Brush in Adobe After Effects. This 3D reconstruction technique is ideally suited for tissues that are too large for ***ysis by Confocal Microscopy but too small for MRI or PET/CT. In addition, the cost of ***ysis is negligible, since it uses a consumer-grade camera on a conventional light microscope and readily available software by Adobe. For more examples of 3D reconstruction using this technique visit: www.ars.usda.gov For more information on diagnosis of liver diseases in dogs and cats, using both histological and clinical criteria, see the monograph: "WSAVA Standards for Clinical and Histological Diagnosis of Canine and Feline Liver ...
Reproductive Organs and Cells A tour of histological features of the mammalian male and female reproductive organs, utilizing human and domesticated animal tissue. For more information on cells, tissue and organs in general, visit
Cattle Decapitation - Polyps Lyrics: An onset of polyps Spurt from the rectum Like a bouquet of red fingers Painful, yet gorgeous So, this is what you get for this life that you've led Your bloomin' tulips turning red. Pulsating Pappular Protuberance Sessile villous adenoma Deep inside we can see with colonoscopy Stalactitical growths from hardened adipose Formations of bags contain polypular tissue Bursting open, causing anemia Histological tests reveal the presence of cancer Could have been avoided, somebody made a big mistake Substandard diet, consumption of meat led to this disease Atypial polyps bringing you to your knees Polypectomy On total obstructing colorectal cancer Merging tapeworms Make their homes in the polyps Blood, clots, hot enema, dead crotch, worms line the colon Disgusting pig-worms in the polyps Nutrients sucked-broken by solids A flexible sigmoidoscopy Revealing upper GI disease Blood, clots, hot enema, dead crotch, worms in the polyps Your colon shuts down Rectus cavernosa Finger-like polyps Stalactite deposits Caved in, bleeding Time for colostomy Poopshoot re-route To the side Now a bag of excrement Is always strapped to your hip Everyone knows it And when you die, you'll be screaming - Why me. Why me. Why me. This could have been stopped This could have been avoided This is what you get for being alive An onset of polyps Spurt from the colostomy hole This time with maggots And hungry *** mites So, this is what you get for this life that you've led Better off dead. Death by ...
Mammary gland ductal tree reconstruction using ImageJ 3D viewer in Fiji Mammary gland skeleton reconstruction view. The ductal network (yellow branches and blue terminal end points) is shown along with the lymph node volume (in fuchsia). More information in the paper: 3D reconstruction of histological sections: Application to mammary gland tissue, Ignacio Arganda-Carreras, Rodrigo Fernandez-Gonzalez, Arrate Munoz-Barrutia, Carlos Ortiz-De-Solorzano, Microscopy Research and Technique, 2010, in press. Link: www3
Shotgun Histology Ovary
ATP Synthase Animation ATP Synthase the Motor of Life by Nasif Nahle When we talk about the macroscopic world, we define the term structure from an anatomical, histological and morphological viewpoint. However, we often ignore the fact that we have to make a qualitative jump in the progression from macrophysical systems to microphysical systems. When we investigate the system as a whole, we must not limit our knowledge simply to macrophysical parameters which can be measured, as length, distance, volume, etc., but to effective parameters which are further related with electric charges, electromagnetic fields, quantum energy and electrodynamic phenomena. We set in motion a picture of a macrophysical system, ie a plant. We can describe its height, its volume, its weight, the quantity of leaves it has, its color, etc.; however, as we move our observations towards smaller and smaller portions of the macroscopic structure, we arrive at a point at which the macrophysical qualities leap into another realm where the parameters with which we previously classified the living organism no longer have any utility. Of course, the transition is so subtle that many of us succumb to the idea that there is no more physical explanation to describe life. If we understand that there is no boundary, but rather a continuum which links every process in every living system with the Universe, we will not be so easily fooled by the illusion of a separation between the macrophysical world and the microphysical world.
Laparoscopic Appendectomy (Laparoskopik Apendektomi) Appendicitis is one of those fascinating abdominal conditions that have a variety of presentations and also there are other conditions that mimic appendicitis. Appendicitis is the most common intra-abdominal surgical emergency with a lifetime risk of 8.6% for males and 6.7% for females, but the risk of appendectomy is 12% for males and 23.1% for females due to the higher rate of negative or incidental appendectomy in females. Difficulties in diagnosis are likely to be encountered, especially in atypical presentations. These can lead to either an inappropriate delay in surgical treatment or an unnecessary exploratory laparotomy. 15-30% of the removed appendices do not have histological signs of inflammation. Several diagnostic modalities, such as ultrasonography (US), computed tomography (CT) and diagnostic laparoscopy (DL) have been introduced. These tools have several limitations, risks and costs. US is an observer dependent test with low sensitivity rates, whereas CT is less observer dependent but relatively expensive and exposes the patient to a relatively high radiation dose. Radiological evaluation helps the surgeon with the diagnosis and differentiation in nearly 100% of cases. Laparoscopy is another reliable diagnostic tool for appendicitis with the advantage of leading to other diagnoses and the possibility of concomitant therapeutic interventions. Negative appendectomy rates are relatively low : 4-13% in a laparoscopic approach. At present, the utility of DL and ...
Expectation-Maximization driven Level Set Model for Gland Segmentation on Prostate Histology Images EM driven integrated region- and boundary-based level set model is applied for Gland Segmentation of Prostate Histological Image. The region term employs statistical information derived from foreground and background regions to approximate a Gaussian mixture model (GMM). The EM algorithm is then applied for more accurate determination and updating of region statistics. The boundary term is described by a local structure tensor based color gradient which results in better defined boundaries
kidney histology Kidney (renal corpuscle, tubules), first part the clear hole is the tubules second is the renal corpuscle the outer layer the clear part. any questions feel free to ask
Fantastic Voyage Part 1: AutoFill on a synaptic vesicle with placeholder proteins and mock bilayer • As of 2010, scientists can see cells, cell organelles, and even large macromolecular structures with microscopes. We can visualize molecules and a handful macromolecular structures with what I call 'nano-inference' technologies like xray crystallography and NMR. • An algorithm we've developed called AutoFill, pools data from several sources to fill organelle, cell, and histological structure data gathered from microscopes (or computationally generated (a recursive scaling capability of AutoFill itself) ), with molecular detail including: structure data from the Protein Data Bank, the TransMembrane Protein DataBank, cryo EM databases, transcriptomes, translatomes (providing rough molecular weight for appropriate sized placeholder spheres), and many other databases. HISTORY • For over a decade, I've contemplated algorithms to help model the interface of these two resolution limits, aka the meso-scale that David Goodsell mgl.scripps.edu has illustrated in watercolored drawings with meticulous research and comprehensive detail for two decades now. • In 2004 I motivated to develop these algorithms. Lacking substantial programming education and experience, I moved in '05 to work next to David in Art Olson's Molecular Graphics Lab at the Scripps Research Institute to formally initiate this project as part of my PhD thesis. While working on other projects, I roughed together several crude versions of the the fundamental algorithms and received valuable feedback from my PI (Art ...
Anaglyph video of Liver Portal Tracts From a Normal and Abnormal Dog You will need red-blue (green) glasses to properly view this video. This is the anaglyph version of the video at This video compares the hepatic portal track of a normal dog's liver to a dog with a congenital vascular abnormality termed a portosystemic shunt. In this condition the portal vein has an abnormal connection to the systemic venous system and deprives the liver of the normal portal blood flow. Red=hepatic arteries, Blue=portal vein, Green=bile duct, Yellow=lymphatic space. In the normal portal tract the size and relationship of the portal vein, the hepatic artery, bile ducts and the lymphatic spaces can be seen. The hepatic portal tract from the dog with the portosystemic shunt is characterized by the lack of a portal vein, dramatically distended lymphatic space and an increased size with multiple small branches of the hepatic artery. This negative-space filling technique was performed on a small number of sections (normal dog=40, shunt dog=48) from FAA-fixed, paraffin-embedded liver tissue. Paraffin blocks were sectioned at 10 microns and treated with a standard H&E stain. Sections were photographed with a Sony DSC707 consumer-grade camera on a Nikon Eclipse 50i at 200X. JPEG images were imported into Adobe After Effects on a Macintosh desk-top computer. Details of the process used to build the 3D volumes are described in a recently published article: www.ars.usda.gov and in our other video: and The 3D images are not cartoons but ...
Recurrence of polyps of the cervix. Radical removal of polyps. In early 2010, steel mill worker, 49 of age was removed polyp of the cervix by scraping the walls of the cervical c***. Histological examination revealed fragments of scraping glandular fibrous and glandular polyps have delivered the material. During regular preventive gynecological examination 6 months later the woman showed recurrent polyps of the cervix. The patient was sent to outpatient polypectomy by endoscopic method. At endoscopy the cervical c*** there were revealed two polyps. Glandular-fibrous polyp was located in the lower third of the cervical c*** and the glandular polyp - in the middle third of the channel. Both polyps were removed by electrosurgical endoscopic method using a loop. Eschar whitish-yellowish after removal remained on the location of each polyp.
On Uranium Mining This video covers the topic of cancer rates around uranium mines. Karl Grossman, former op-ed columnist and author (Cover Up: What Your Not Supposed to Know About Nuclear Power), makes the claim that lung cancer rates are elevated around uranium mines. Gywneth Cravens, author (The Power To Save The World: The Truth about Nuclear Energy), recalls her experiences living in New Mexico, and claims that is not the case. References: 1. Cancer mortality in a Texas county with prior uranium mining and milling activities, 19502001 John D Boice Jr et al 2003 J. Radiol. Prot. 23 247-262 doi: 10.1088/0952-4746/23/3/302 2. Differences in Cancer Incidence among Indians in Alaska and New Mexico and US Whites, 1993-2002 Janet J. Kelly1, Anne P. Lanier1, Steven Alberts2 and Charles L. Wiggins3 1 Office of Alaska Native Health Research, Community Health Services, Alaska Native Tribal Health Consortium, Anchorage, Alaska; 2 Department of Oncology, Mayo Clinic, Rochester, Minnesota; and 3 Epidemiology and Cancer Control, Cancer Research and Treatment Center, University of New Mexico, Albuquerque, New Mexico 3. Radiation, Smoking, and Lung Cancer A binational study provides new insights into the effects of smoking and radiation exposure on different histological types of lung cancer. by Kiyohiko Mabuchi, RERF Department of Epidemiology, Charles E Land, RERF consultant, and health statistician, US National Cancer Institute, Radiation Epidemiology Branch, Bethesda, Maryland, and Suminori Akiba ...
Acts9.26-43:Saul came to Jerusalem to join His Twin Brethren Act 9.26-43:- And when Saul was come to Jerusalem, he assayed to join himself to the Labourers: but they were all afraid of him, and believed not that he was a Labourer. 27 But Barnabas took him, and brought him to the apostles, and declared unto them how he had seen the Lord in the way, and that he had spoken to him, and how he had preached boldly at Damascus in the name of Jesus. 28 And he was with them coming in and going out at Jerusalem. 29 And he spake boldly in the name of the Lord Jesus, and disputed against the Grecians: but they went about to slay him. 30 Which when the brethren knew, they brought him down to Caesarea, and sent him forth to Tarsus. 31 Then had the churches rest throughout all Judaea and Galilee and Samaria, and were edified; and walking in the fear of the Lord, and in the comfort of the Holy Ghost, were multiplied. 32 And it came to pass, as Peter passed throughout all quarters, he came down also to the saints which dwelt at Lydda. 33 And there he found a certain man named Aeneas, which had kept his bed eight years, and was sick of the palsy. 34 And Peter said unto him, Aeneas, Jesus Christ maketh thee whole: arise, and make thy bed. And he arose immediately. 35 And all that dwelt at Lydda and Saron saw him, and turned to the Lord. 36 Now there was at Joppa a certain Labourer named Tabitha, which by interpretation is called Dorcas: this woman was full of good works and almsdeeds which she did. 37 And it came to pass in those days, that she was ...
Hyperbaric Oxygen Therapy for Reduction of Secondary Brain Damage in Head Injury http Cerebral contusions are one the most frequent traumatic lesions and the most common indication for secondary surgical decompression. The purpose of this study was to investigate the physiology of perilesional secondary brain damage and evaluate the value of hyperbaric oxygen therapy (HBOT) in the treatment of these lesions. Five groups of five Sprague-Dawley rats each were submitted to dynamic cortical deformation (DCD) induced by negative pressure applied to the cortex. Cerebral lesions produced by DCD at the vacuum site proved to be reproducible. The study protocol entailed the following: (1) DCD alone, (2) DCD and HBOT, (3) DCD and post-operative hypoxia and HBOT, (4) DCD, post-operative hypoxia and HBOT, and (5) DCD and normobaric hyperoxia. Animals were sacrificed after 4 days. Histological sections showed localized gross tissue loss in the cortex at injury site, along with hemorrhage. In all cases, the severity of secondary brain damage was assessed by counting the number of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) and caspase 3-positive cells in successive perilesional layers, each 0.5 mm thick. Perilesional TUNEL positive cells suggested the involvement of apoptosis in group 1 (12.24% of positive cells in layer 1). These findings were significantly enhanced by post-operative hypoxia (31.75%, p 0.001). HBOT significantly reduced the severity and extent of secondary brain damage expressed by the ...
Histological Tooth Development
A novel histological scoring system to evaluate mucosal biopsies from patients with eosinophilic... Dr. James Lee discusses his m***cript A Novel Histologic Scoring System to Evaluate Mucosal Biopsies From Patients With Eosinophilic Esophagitis. To view the print version of this abstract go to
Histological Structure of Digestive System Organs Pictures taken in A&P lab.
Shotgun Histology Small Intestine Ileum
Bridging the Gap between Cortical Structure and Cortical Fun Almut Schüz, Prof. Dr. Research Scientist My research area is the structure of the brain, with the aim to connect brain structures to brain functions (eg learning, thinking, orientation selectivity). The focus of my research is on the cerebral cortex; comparison with other parts of the brain plays, however, an important role in this. Mainly histological methods are used, in particular quantitative neuroanatomical methods.
3D reconstruction of Gymnotus coropinae 3D reconstruction of the electric organ of Gymnotus coropinae. www.BioVis3 Special thanks to María E. Castelló, Alejo Rodríguez-Cattáneo, Pedro A. Aguilera, Leticia Iribarne, Ana Carolina Pereira and Ángel A. Caputi
Histology - Electron Micrography of the Heart Tissues Part 1 Гистология человека - Сердечная мышечная ткань A practical Histology lesson devoted to the histological structure of the heart.Explanation in English by our beloved teacher Ljudmila Alekseevna.
High-throughput system for detecting cancerous regions in prostate histological sections This is a demonstration of our high-throughput system for detecting cancerous regions in histological sections from radical prostatectomies using probabilistic pairwise Markov models, a novel type of Markov random field (MRF). The basic algorithm proceeds as follows: Step 1) The glands (or, more precisely, the gland lumens) are identified and segmented. Step 2) Morphological features are extracted from the segmented boundaries. Step 3) A Bayesian classifier assigns a probability of malignancy to each gland. If the probability exceeds a predetermined threshold the gland is labeled as malignant; otherwise it is labeled benign. Step 4) An MRF algorithm (iterated conditional modes) refines these labels, thereby enforcing the precept that nearby glands tend to share the same class. Step 5) The cancerous glands are consolidated into continuous regions.
Shotgun Histology Spleen
Shotgun Histology Placenta
Ayurvedic Medicine For Optimizing Cardiovascular Health Contributed by: (www.india-) Cardiovascular disease refers to the class of diseases that involve the heart and blood vessels (arteries and veins). While the term technically refers to any disease that affects the cardiovascular system, it is usually used to refer to those related to atherosclerosis (arterial disease). These conditions have similar causes, mechanisms, and treatments. In practice, cardiovascular disease is treated by cardiologists, thoracic surgeons, vascular surgeons, neurologists, and interventional radiologists, depending on the organ system that is being treated. There is considerable overlap in the specialties, and it is common for certain procedures to be performed by different types of specialists in the same hospital. Most Western countries face high and increasing rates of cardiovascular disease. Each year, heart disease kills more Americans than cancer. Diseases of the heart alone caused 30% of all deaths, with other diseases of the cardiovascular system causing substantial further death and disability. It is the number 1 cause of death and disability in the United States and most European countries. A large histological study (PDAY) showed vascular injury accumulates from adolescence, making primary prevention efforts necessary from childhood. By the time that heart problems are detected, the underlying cause (atherosclerosis) is usually quite advanced, having progressed for decades. There is therefore increased emphasis on preventing ...
Dr. Alan Faden: University of Maryland School of Medicine Faculty Profile Dr. Alan Faden is professor of Anesthesiology at the University of Maryland School of Medicine an Director, Center for Shock, Trauma and Anesthesiology Research (STAR) & National Study Center for Shock and EMS. Dr. Faden's research focuses on mechanisms and modulation of cell death and neuroinflammation after experimental brain or spinal cord injury, including molecular and cell biology, animal modeling, behavior and drug discovery. The program is highly interdisciplinary, utilizing molecular and cellular biology, biochemistry, electrophysiology, pharmacology, behavior, magnetic resonance imaging and spectroscopy, and quantitative histological ***ysis.
Management of Liver Hemangioma Female, 34 years, 10 days after birth. Tumor in the right abdomen was found. Appendicular infiltrate was suspected. CT and ultrasound were made. Results were contradictory - kidney tumor, colon tumor. Laparoscopy was made. Liver tumor was found. Histological conclusion - Liver TB. Repeated histological conclusion - hepatocellular carcinoma. Histology re-examined, the conclusion - non-specific changes. Who said that histological examination gives answers to all questions? Our opinion, given the macroscopic structure of tumor, it is hemangioma or cyst of liver.
3D reconstruction of mice airways using ImageJ 3D viewer Model volume generated from x-ray tomography of mice airways to validate the reconstruction method in the paper: 3D reconstruction of histological sections: Application to mammary gland tissue, Ignacio Arganda-Carreras, Rodrigo Fernandez-Gonzalez, Arrate Munoz-Barrutia, Carlos Ortiz-De-Solorzano, Microscopy Research and Technique, 2010, in press. (www3 Volume size: 444x471x568 pixels.
Hepatology. Hepatitis C. Histological examination. m.nih.gov
BioVis3D Demonstration BioVis3D ( www.BioVis3 ) is 3D reconstruction software for spacial visualization and ***ysis, specifically designed for biologists and researchers who work with serial section slides.
Histological view of Kidney Scanning View ofGlomerulus, Proximal & Distal Tubules and collecting duct of the Kidney for Exam 4
Shotgun Histology Kidney
Myofascial Trigger points: Introduction Myofascial trigger points (MTrPs) are a common muscular problem. Note that this is different from muscle spasms and general soreness. MTrPs can be classified as being active or latent. active MTrPs can refer pain to close or distant locations in the body. They have many different causes, ranging from trauma to metabolic. These points can be treated by a trained professional, and may even be treated by self-manual therapy. New imaging such as ultrasound, MRE, Electromyography, as well as histological and biochemical ***ysis of these MTrPs has recently given insight to their nature.
Histology of the Tooth Junctional Epithelium.MOV A brief description of the role of the Junctional Epithelium and their histological structure.
Histology - Electron Micrography of the Heart Tissues Part 2 Гистология человека - Сердечная мышечная ткань A practical Histology lesson devoted to the histological structure of the heart.Explanation in English by our beloved teacher Ljudmila Alekseevna.
FUSION RF - ENGLISH SPEECH Fusion RF is a non-invasive skin tightening and lipo-dissolving treatment using radiofrequency energy, which uniformly heat the dermis. It works by producing heat when the tissues resistance converts the electric current to thermal energy deeper within the dermis. The radiofrequency depends on the typical resistance of the tissues, therefore a high impedance tissue, such as the adipose tissue, generates less heat and the thermic effect is lower. The increased temperature in the dermis causes collagen contraction, realignment and new collagen production. It is used to treat skin laxity, resulting in firmer, younger looking skin. Heat induces fat cell elimination reducing the fat layer; it increases blood circulation to the area resulting in the drainage of the fatty deposits and toxins through the lymphatic system. After the first treatment the skin will be already smoother and more compact. Histological exams have also demonstrated that RF, besides stimulates new collagen growth, causes a reduction of the dimension of sebaceous gland. For this reason RF is successfully used in the treatment of acne. The reduction is over 75%. The treatment is absolutely non invasive. The patient will experience a brief, deep heating sensation as the RF energy is delivered to the skin and underlying tissue. This deep heating sensation is patients indication that collagen is reaching effective temperatures for tightening. The epidermis (outer layer of skin) is protected by a built-in ...
Shotgun Histology Colon
DECOMPRESSION / AXIAL TRACTION USED TO BE A TORTURE DEVICE THE RACK A REVIEW OF PEER REVIEWED LITERATURE OF THE HISTOLOGICAL CHANGES IN INTERVERTEBRAL DISCS OF THE LUMBAR SPINE SHOWS SERIOUS DEGENERATION OF THE TISSUES. Back in the Spanish Inquisition, the Church used "axial traction" applied to alleged heretics. It's nickname was "the Rack". A due diligent search of the Inquisitor's records does not show that anyone screamed out "Yes, I have committed heresy, and the pain is horrible, but (smiling) my lumbar disc mysteriously feels great!". Nothing in the referenced 1990 article in the Journal of Bone and Joint Surgery by the Japanese authors, even hints that possibly, these crumbling, dried out, worn out tissues, would be made new again by axial traction (commonly known as stretching), and there has not been any journal article I have found that senescent tissues have become young, vital, hydrated and hydrophilic again, and I shall probably bring more recent articles forward that describe the irreversible disintegration of the anulus fibrosus and nucleus pulposus. Take a worn out crumbling rubber band, "stretchy stretchy stretchy" and it will "breaky". There is no magic in stretching that will act like the Philosopher's Stone or Mystical Alchemy and make worn out, old dessicated and friable tissues, and resore elasticity and youth to them. We have found NO Exilir of Youth, no Ponce De Leon potable in old fashioned axial traction, but apparently, axial traction was good for one thing, getting people to confess to being witches.
Shotgun Histology Pharyngeal Tonsil