SENS4 - Manipulation and Derivation of the Hematopoietic Stem Cell Niche (1/3) Manipulation and derivation of the hematopoietic stem cell niche.
Hematopoietic and mesenchymal stem cells into pancreas for more info see
Crawling Neutrophil chasing bacterium (Fagocitando bacteria) Crawling Neutrophil chasing a Bacterium (Un glóbulo blanco persigue a una bacteria) White blood cells, or leukocytes, are cells of the immune system defending the body against both infectious disease and foreign materials. Several different and diverse types of leukocytes exist, but they are all produced and derived from a multipotent cell in the bone marrow known as a hematopoietic stem cell. Leukocytes are found throughout the body, including the blood and lymphatic system. The number of leukocytes in the blood is often an indicator of disease. There are normally between 4×109 and 11×109 white blood cells in a litre of blood, making up approximately 1% of blood in a healthy adult. In conditions such as leukemia, the number of leukocytes is higher than normal, and in leukopenia, this number is much lower. The physical properties of leukocytes, such as volume, conductivity, and granularity, may change due to activation, the presence of immature cells, or the presence of malignant leukocytes in leukemia. Neutrophil Neutrophils defend against bacterial or fungal infection and other very small inflammatory processes that are usually first responders to microbial infection; their activity and death in large numbers forms pus. They are also known as polymorphonuclear leukocytes. They have a multilobed nucleus which may appear like multiple nuclei, hence the name polymorphonuclear leukocyte. The cytoplasm may look transparent because of fine granules that are faintly pink in ...
Stem Cell Types and Sources
Hematopoietic stem cell transplantation #2 Bone Marrow Transplantation (or Hematopoietic Stem Cell Transplantation) This modern procedure is the only accepted method worldwide to cure beta-thalassemia diseases. The patients will need to have suitable HLA-matched healthy donors, possibly their own brothers / sisters or unrelated volunteers. We will test the patients blood, as well as potential donors, for the typing and matching and cooperate in circumstance of searching for appropriate unrelated donor is needed. Sources of donors stem cells may be achieved from bone marrow, peripheral blood, or umbilical cord blood. Allogeneic bone marrow transplantation is sophisticated scheme of therapy and integration of medical sciences and technology. We offer this well-organized comprehensive treatment programs for particular group of patients who have full-filled indication for undergoing transplantations. Our hematologist will discuss, explain and answer you in details regarding this effective and informed curative therapy.
CAL-101 in CLL chronic lymphocytic leukemia TARGETED AGENT SHOWS PROMISE FOR CHRONIC LYMPHOID LEUKEMIA For more information: CLL: www.leukemia- An abstract of the study: m.nih.gov Researchers at the Ohio State University Comprehensive Cancer Center-Arthur G. James Cancer Hospital and Richard J. Solove Research Institute (OSUCCC-James) have identified an experimental agent that targets chronic lymphocytic leukemia and perhaps other proliferative disorders of lymphocytes. Their study shows that the small-molecule inhibitor CAL-101 directly promotes cell death by apoptosis in chronic lymphocytic leukemia (CLL) cells and disrupts several external survival pathways needed for CLL cell viability and proliferation. The agent blocks a molecule called PI3K-delta, an isomer of the PI3K (phosphatidylinositol-3 kinase) pathway, which is activated mainly in blood-forming, or hematopoietic, cells. The research was posted recently in the journal Blood. "Overall, our findings provide a rationale for the development of CAL-101 as the first in a new class of targeted therapies for CLL," says principal investigator Amy J. Johnson, assistant professor of hematology and medicinal chemistry, and a CLL researcher in the OSUCCC-James. "A PI3K inhibitor hasn't been developed yet because this pathway is required for many essential cellular functions, but the identification of PI3K-delta, which is hematopoietic-selective, unlocks a potential new therapy for B-cell malignancies," Johnson says. CLL is the most common ...
Stem Cells and Beyond Part 2 A projcect for immunolgy about hematopoietic stem cells, part 2 of 2
Benzene Is A Carcinogen Found In Gasoline / Chemical Safety PSA Benzene Is A Carcinogen Found In Gasoline / Chemical Safety PSA. Public domain video courtesy of NIH. Gasoline contains benzene, depending on the source of gasoline, and countries, ranges of concentration of benzene in gasoline have been reported as 1 to 6%. Gasoline is also used as an industrial solvent and workers commonly experience inhalation and skin exposure. In my practice, it is not unusual to hear from workers that "I washed my hands with gasoline to remove paint daily" or "the smell was so strong that I got dizzy", demonstrating substantial exposure to gasoline ongoing on a daily basis. Since benzene is a known human carcinogen, it would be expected that gasoline will be also a hematopoietic toxic and cancer causing agent as well. Indeed, Aksey et al as early as 1928 reported aplastic anemia, and in 1941, Machele et al reported thrombocytopenia from gasoline intoxication. Other hematopoietic malignancies have been reported as a result of gasoline exposure. Epidemiological studies of workers and filling station attendees have shown genotoxic effects at very low benzene from gasoline vapor exposure. Brandt et al have demonstrated genotoxic effects in workers exposed to low levels of benzene from gasoline. Santos-Mello et al have shown chromosomal deletions in lymphocytes of workers exposed to gasoline as attendants. Infante et al reported hematopoietic malignancy in petrol exposed workers. Similar exposures to gasoline in garage mechanics and filling stations have ...
Bone Marrow Stem Cells
Gene therapy improves stem cell transplantation Dutch tv journal of 15 June 2006 about new method to prevent immune reactions after cell transplantation. Suicide genes form the basis of a strategy for making cancer cells more vulnerable, more sensitive to chemotherapy. The approach has been to attach parts of genes expressed in cancer cells to other genes for enzymes not found in mammals that can convert a harmless substance into one that is toxic to the tumor. Most suicide genes mediate this sensitivity by coding for viral or bacterial enzymes that convert an inactive drug into toxic antimetabolites that inhibit the synthesis of nucleic acid. Suicide genes must be introduced into cells in ways that ensure their uptake and expression by as many cancer cells as possible, while limiting their expression by normal cells. Suicide gene therapy for cancer requires the vector to have the capacity to discriminate between target and nontarget cells, between the cancer cells and normal cells. acute lymphocytic leukemia, acute myelogenous leukemia, acute nonlymphocytic leukemia, ALL, AML, ANLL, anemia, blast crisis, blasts, blood, bone marrow, cancer, chronic lymphocytic leukemia, chronic myelogenous leukemia, CLL, CML, erythrocytes, granulocytes, hematopoiesis, hematopoietic stem cells, leukocytes, lymphoid lineage, lymphoid stem cells, myelocytic leukemia, myeloid lineage, myeloid stem cells, platelets, red blood cells, RBCs, thrombocytopenia, white blood cells, WBCs, hairy cell leukemia, human T-cell leukemia, chemotherapy ...
Blood circulation in caudal vein plexus in zerbrafish Murayama E et al . Tracing Hematopoietic Precursor Migration to Successive Hematopoietic Organs during Zebrafish Development, ImmunityVolume 25, Issue 6, , December 2006, Pages 963-975.
Procedure for Setting Up the CFC Assay This instructional video provides step-by-step guidelines for setting up the colony-forming cell (CFC) assay using MethoCult® from STEMCELL Technologies (bit.ly Steps covered include preparing MethoCult®, preparing cells, adding cells to MethoCult®, and plating and incubating.
David Baltimore (Cal Tech) Part 3: The Grand Challenge: Engineering Immunity In this last segment, I describe another gene therapy strategy for HIV in which we propose to develop antibody-like proteins that can be expressed by a patient's B cells and will target the HIV virus for destruction. To achieve this objective, hematopoietic (blood) stem cells must to be targeted with the gene, which will ultimately develop into B cells that express the therapeutic molecule. The ultimate goal is to produce a life-long supply of anti-HIV neutralizing antibodies. In this lecture, I describe the molecular methods underlying this strategy and a development path from proof-of-principle studies in mouse to safe trials in humans. This project receives funding from the Bill and Melinda Gates Foundation. See more at
Dance Your Dissertation 2010 - Daria Neidre The Use of Autologous Adipose and Bone Marrow Derived Stem Cells in a Point of Care Goat Non-Instrumented Posterolateral Lumbar There are over 185, 000 spinal fusions performed each year. The gold standard for these fusions is the Iliac Crest Bone Graft (ICBG) where a potion of the bone is harvested from the iliac crest and implanted into the lumbar fusion site. This procedure has many complications including donor site necrosis, donor site morbidity and long-term pain and discomfort for the patient. An alternative to ICBG is harvesting bone marrow through iliac crest aspiration, which contains adult mesenchymal stem cells (BMSC) that facilitate bone growth when placed on an appropriate bone-like scaffold. Recently, adult stem cells derived from adipose (fat) tissue (ADSC) have demonstrated similar abilities of bone growth to those isolated from the bone marrow. Reserves for isolation of BMSCs are small due to the limited volume of marrow within the iliac crest. Adipose tissue has demonstrated easier access of tissue, higher stem cell number yield, and larger amounts of tissue to isolate stem cells, without the problems associated with BMSC and ICBG. We aimed to characterize and compare in vitro stem cells isolated from bone marrow and fat tissue and in vivo implantation in a non-instrumented posterolateral spinal fusion in a goat large animal model as a human translational study. The goat model is a common large animal model for orthopaedics as they are similar to humans ...
Zebrafish hematopoiesis: the definitive wave A brief animation describing the induction of hematopoietic stem cells in the zebrafish.
MethoCult® Express for Cord Blood Banks and Transplantation Centers An innovative methylcellulose-based medium specifically formulated to allow enumeration of total hematopoietic progenitors (CFC) in cord blood units in only 7 days.
"Immune" Documentary - Part 2 - Organs and Hematopoiesis Part 2 of "Immune" (See Part 1 for additional info)
Induction of hematopoietic stem cells in zebrafish This animation shows the different steps of hematopoiesis. Blood flow is required for the initial induction of hematopoietic stem cells, as shown in a paper by North et al. Cell. 2009 May 15;137(4):736-48.
Granulocyte in the Caudal Hematopoeitic Tissue at 4 dpf Murayama E, et al Tracing Hematopoietic Precursor Migration to Successive Hematopoietic Organs during Zebrafish Development, ImmunityVolume 25, Issue 6, , December 2006, Pages 963-975.
STEMvision™ Automated and Standardized CFC Enumeration STEMvision™ - - is the world's first automated instrument and computer system for imaging and scoring hematopoietic colonies in the colony-forming cell (CFC) assay. This video introduces the features and benefits of the STEMvision™ system.
Margination and Rolling of Hematopoeitic Progenitors in the Murayama E, et al Tracing Hematopoietic Precursor Migration to Successive Hematopoietic Organs during Zebrafish Development, ImmunityVolume 25, Issue 6, , December 2006, Pages 963-975
Lymplex Lymph Node Cleansing Part 2 "The transformation of primitive or immature lymphocytes into T-lymphocytes and their proliferation in the lymph nodes is promoted by a thymic hormone called thymosin. Ocassionally the thymus persists and may become cancerous after puberty and and the continued secretion of thymosin and the production of abnormal T-cells may contribute to some autoimmune disorders. Conversely, lack of thymosin may also allow inadequate immunologic surveillance and thymosin has been used experimentally to stimulate T-lymphocyte proliferation to fight lymphoma and other cancers. 4. The spleen: The spleen filters the blood and reacts immunologically to blood-borne antigens. This is both a morphologic (physical) and physiologic process. In addition to large numbers of lymphocytes the spleen contains specialized vascular spaces, a meshwork of reticular cells and fibers, and a rich supply of macrophages which monitor the blood. Connective tissue forms a capsule and trabeculae which contain myofibroblasts, which are contractile. The human spleen holds relatively little blood compared to other mammals, but it has the capacity for contraction to release this blood into the circulation during anoxic stress. White pulp in the spleen contains lymphocytes and is equivalent to other lymph tissue, while red pulp contains large numbers of red blood cells that it filters and degrades. The spleen functions in both immune and hematopoietic ...
MS Cure - Progression & Recovery Following Stem Cell Transplant There is only one scientifically established "cure" for Multiple Sclerosis that is available. It is a stem cell transplant (not to be confused with "stem cell therapy"). Please visit my blog at: This video descibes the mechanism of MS recovery & improvement (with deficit reversal) following the cure of a stem cell transplant procedure.
Living with Lupus: Hematopoietic Stem Cell Therapy for Lupus For the full video, click here: Centricity Series website: Molecular Medicine website: Feinstein Institute website: North Shore-LIJ website:
Stem Health Hellas - Hematopoietic Progenitor Cells
Insidermedicine in 60 - April 14, 2009 From Vancouver - According to research in the Canadian Medical Association Journal, smoking both tobacco and marijuana increases the risk of chronic obstructive pulmonary disease. In a study of nearly 900 people aged 40 and over, researchers found that those who smoked tobacco and marijuana had a 3 times higher risk for COPD. However, an increased risk was not linked to smoking marijuana alone. From Brazil - According to research in the Journal of the American Medical Association, stem cells may be effective for type 1 diabetes. In a study of 23 patients, researchers found that 20 were able to stop taking insulin after they underwent hematopoietic stem cell transplantation. After 2 years, only 8 of the patients relapsed, and even then required only low doses of insulin. And finally, from San Francisco - According to research in the Archives of Internal Medicine, prison time may increase cardiovascular risk. In a study of over 4300 adults, researchers found that those who were incarcerated were more likely to have hypertension and left ventricular hypertrophy than those who were not. Drug and alcohol use, obesity, lower socioeconomic status or increased stress could be the cause. For Insidermedicine in 60, I'm Allison Chow from Queen's University.
Hematopoietic stem cells 3 of 9 Basic explanation of hematopoietic stem cells and why they are important.
SENS4 - Manipulation and Derivation of the Hematopoietic Stem Cell Niche (2/3) Manipulation and derivation of the hematopoietic stem cell niche.
Bone marrow transplantation HD, ENG subtitles HD Movie made for: Clip showing both methods of bone marrow [or Hematopoietic Stem Cells - HSC] transplantation. The movie was made in Poland, but I've added professional English subs, so that You can watch it too... follow me!
Bone Marrow Producing Insulin a description of the publication In vivo and in vitro characterization of insulin-producing cells obtained from murine bone marrow. Diabetes. 2004 Jul;53(7):1721-32
City of Hope's Bone Marrow Transplant Program (BMT) with Stephen J. Forman, MD Stephen J. Forman, MD, chair of hematology and hematopoietic cell transplantation, shares his views on the essence of care at City of Hope. He highlights the bone marrow transplant program (BMT) and the programs growth over the years. The video includes footage of the annual BMT Reunion and what the event means to the City of Hope team and to the patients.
Part 1: Hematopoietic stem cell transplant Part 1 of 2: Video discussion between Dr. Harry S. Jacob and Dr. Keith Sullivan about hematopoietic stem cell transplant.
Brian's Stem Cell/Chemo Recovery Page from MS Videos of my recovery from Hematopoietic Stem Cell Transplant procedure.
CheckOrphan NewsFlash November 3, 2009 CheckOrphan NewsFlash for Tuesday, November 3, 2009 - breaking news about rare diseases, orphan diseases, orphan drugs, and neglected diseases. Today's broadcast highlights topics about: Huntington's disease, pancreatic cancer, Epstein-Barr virus, Friedreich's ataxia, Nipah virus, Hendra virus, systemic lupus, flu, H1N1, Caltech, Htt protein, Happ1, immunotherapy, hematopoietic stem cell, Clavis Pharma, BENLYSTA, CSIRO, Lundbeck, GlaxoSmithKline, antioxidants, intrabody, glutamine, flu prevention, lymphoma, huntingtin, Htt, Happ1, CTL treatment, HSCT, Orphan Drug Designation http
SENS4 - Manipulation and Derivation of the Hematopoietic Stem Cell Niche (3/3) Manipulation and derivation of the hematopoietic stem cell niche.
Evidence of Stem Cell Benefits
Stem Cells Stem cells serve as the source of all the other specialized cells in the body, both when it forms during embryogenesis and when it replaces cells that have been lost to aging, injury or disease.
Gleevecs mechanism of Action The animation begins by introducing the Philadelphia Chromosome, the result of a reciprocal translocation between chromosomes 9 and 22. More specifically the breakpoint cluster region (BCR) of chromosome 22 is fused with part of the Abelson (ABL) gene on chromosome 9. The resulting BCR-ABL genetic domain now located within chromosome 22 and codes for a mutant tyrosine kinase also known as BCR-ABL. Under normal circumstances tyrosine kinase proteins respond to external cellular messaging proteins, and ultimately initiate a series of reactions that culminate in cellular replication. Conversely, BCR-ABL is constitutively active, meaning it does not require activation by the aforementioned cellular messaging proteins in order to stimulate cellular replication. This results in acceleration of cell division, an inhibition of DNA repair, overall genomic instability, and the fatal blast crisis characteristic of chronic myelogenous leukemia. The animation progresses to introduce Gleevec (imatinib), the first in a class of drugs that specifically target and competively inhibit the ATP binding site on BCR-ABL tyrosine kinase. This prevents the ABL domain from phosphorylating the tyrosine residue, and as a result preventing the proliferation of hematopoietic cells that express BCR-ABL. Therapy with imatinib results in a dramatic reduction of tumor clone cells and the occurrence of blast crisis', through targeted drug treatment which leaves health cells unscathed. Created by: Nelson ...
Haematopoietic cell precursor at Caudal vein plexus Murayama E, et al Tracing Hematopoietic Precursor Migration to Successive Hematopoietic Organs during Zebrafish Development, ImmunityVolume 25, Issue 6, , December 2006, Pages 963-975.
Part 2: Hematopoietic stem cell transplant Part 2 of 2: Video discussion between Dr. Harry S. Jacob and Dr. Keith Sullivan about hematopoietic stem cell transplant.