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explants

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Images

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  • WG0321 G were immunostained with monoclonal anti vimentin V9 antibody and Oil Red O Vimentin filaments were stained in green and lipid droplets were stained in red Scale bars 10 μ m
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  • vimentin and keratins Both proteins could be found in the aggregates white arrows Note that some vimentin aggregates did not contain keratins arrowheads in D Scale bar 10 μ m Figure 3 Quantitative RT PCR ***yses of the GAN and lipid metabolism related genes in fibroblast explants grown in low serum conditions
  • Figure 2 Suppression of adiponectin release from abdominal SC adipose explants by BPA E2 and ICI A Typical dose response by explants from one patient each value is the mean
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  • Tissue Culture Stage 2 Explants
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  • Embryogenic response was only obtained in explants cultured in picloram figure 11 and dicamba
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  • Table 1
  • Figure 4 Testosterone production in vitro by fetal human testis explants after culture for 24 hr at 37°C with or without the addition of hCG 22R CHO or KTZ in the presence or
  • Figure 1 a and b are the main cell types found in the calli a Non dividing cells with small nuclei nm and small meristematic m cells with densely stained cytoplasm
  • Figure 6 Leydig cell parameters in neonatal marmoset co twins treated for 14 days with either vehicle control twin or 500 mg MBP kg day A Leydig cell volume testis B Average
  • Factors that regulate nerve growth can be tested by coculturing
  • Figure 3 BPA and E2 suppress adiponectin release from mature abdominal SC adipocytes from a nonobese woman A and an obese woman B A Effect of treatment with increasing
  • Table 3
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  • formed roots Maximum embryogenesis obtained was 80 2 with 2 68 m M NAA and 22 19 m M BAP combination which was statistically significant at 1 level Using cotyle Figure 1 a c Plantlet regeneration by somatic embryogenesis from groundnut mature cotyledon explants a Mature cotyledon explant exhibiting somatic embryogenesis on
  • Microtome Chuck Use the Orientation Handle to rock the specimen so that its PITCH is parallel to the knife This should produce good cross sections Leaf explants should be oriented parallel to the knife edge for cross sections This may not be a concern for stems and roots since they tend to be cylindrical Lock the stub in
  • Blocks of human tissues were prepared and infected with the R5 HIV 1 strain SF162 and cultured as described in the protocol The culture media were collected and replaced at different time
  • The expression of each gene in MCH070 WG0791 and WG0321 cells was compared to that in MCH068 cells Each data point is the mean of three separate runs GAPDH was used for normalization Figure 4 Cytological staining of normal and GAN fibroblasts grown under low serum conditions A Percentage of cells containing Red Oil
  • Figure 5 Depot specific differences in the expression of putative receptors that may mediate the action of BPA or E2 as determined by real time reverse transcriptase PCR A
  • Figure 1 Suppression of adiponectin release from breast adipose explants by BPA E2 and ICI A Typical dose response by explants from one patient each value is the mean ± SE of
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  • Figure 3 Testosterone production in vitro by GD19 5 rat testis explants after culture for 48 hr at 37°C with or without the addition of hCG 22R CHO or KTZ in the presence or
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  • 9 is included in the mRNA gray box The intronic mutation in WG0791 is indicated with an arrowhead The positions of the RT PCR primers used to amplify GAN cDNA are also shown arrows Figure 2 Cytological ***ysis of normal and GAN fibroblasts A Fractions of wild type and GAN cells containing vimentin aggregates in
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  • In subsequent stages somatic embryos were observed developing from the embryogenic regions These were morphologically similar to zygotic embryos of Musa balbisiana
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  • Los resultados que obtuvieron indican que el procedimiento de recolección de los explantes debe ser el uso del antioxidante a baja temperatura o el uso del sombreamiento Conclusiones y recomendaciones
  • Evaluation of the explants at days 15 and 30 in induction medium confirmed the histological observations in the control and NAA treatments table 1 Culturing the explants
  • Description of Research The Group focuses on molecular farming to produce recombinant proteins of interest in human health biofuels based on chloroplast genetic engineering and genomics of cotton with emphasis
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Videos

  • Retrograde transport of Alomone Labs mNGF 2.5S-Biotin (#N-240-B) conjugated to Qdot in DRG explants. Retrograde transport of Alomone Labs mNGF 2.5S-Biotin (#N-240-B) conjugated to Qdot in DRG explants. Dorsal root ganglia explant from E13.5 mice were grown 3 days in compartmental chambers. 4 nM of mNGF2.5S-Biotn (#N-240-B) conjugated to Qdot605 were added to the distal axon chamber. Retrograde transport of Qdot-NGF was imaged along one axon. Pictures were taken every 2 sec at x60 magnification.
  • ScienceCasts - Hidden Magnetic Portals Around Earth Hidden Magnetic Portals Around Earth NASA ScienceCasts
  • solving quadratic equations using factorization How to solve quadratic equations, using factorization lesson 1
  • animals in space part one hey guys were making a movie hope you like it leave a com ment and subscribe
  • Generation of Composite Plants in Medicago truncatula used for Nodulation Assays Watch video protocols on JoVE - . Journal of Visualized Experiments (JoVE) is a scientific journal publishing video articles on biological experiments filmed at leading research institutions. JoVE is the first video journal indexed in PubMed. To see more of this protocol go to Generation of Composite Plants in Medicago truncatula used for Nodulation Assays This video protocol was filmed at the Donald Danforth Plant Science Center. Similar to Agrobacterium tumerfaciens, Agrobacterium rhizogenes can transfer foreign DNAs into plant cells based on the autonomous root-inducing (Ri) plasmid. A. rhizogenes can cause hairy root formation on plant tissues and form composite plants after transformation. On these composite plants, some of the regenerated roots are transgenic, carrying the wild type T-DNA and the engineered binary vector; while the shoots are still non-transgenic, serving to provide energy and growth support. These hairy root composite plants will not produce transgenic seeds, but there are a number of important features that make these composite plants very useful in plant research. First, with a broad host range,A. rhizogenes can transform many plant species, especially dicots, allowing genetic engineering in a variety of species. Second, A. rhizogenes infect tissues and explants directly; no tissue cultures prior to transformation is necessary to obtain composite plants, making them ideal for transforming recalcitrant plant species ...
  • Metal on Metal Minute | Preservation of Explants Please visit us at or http for more information. TRANSCRIPT: Hi my name is Sam Davis from the law firm of Davis Saperstein and Salomon and I'd like to welcome you back to another Metal on Metal Minute. Today we're going to talk about preservation: Preservation of your explant. That is the device that was removed and replaced during your surgery because the first hip replacement didn't work and may have been defective. Why is it important for you to think about the preservation of what the doctors have removed from your body? The reason is very simple the explant or joint replacement that was removed from your body, may well be the most important piece of evidence in your case if you are explant has been properly preserved, our experts will be able to tell what the cause of the failure was meaning by looking at the surfaces using great magnification such as an electron microscopes or laser ***ysis of wear patterns we can tell what the nature of the failure was and whether or not you have suffered metallosis or the pseudo tumors that are all too commonly seen in these cases. So how is preservation of a device accomplished? It's done with a certified letter supplemented by either and email or fax sent both to your orthopedic surgeon and to the hospital and its pathology department you don't want to let the manufacturer get their hands on the device unless it's done pursuant to a court order. Well thank you for joining us for this Metal on Metal Minute. My ...
  • Panic continues as Noni spreads Outbreak Noni grows in shady forests as well as on open rocky or sandy shores. It reaches maturity in about 18 months and then yields between 48 kilograms (8.818 lb) of fruit every month throughout the year. It is tolerant of saline soils, drought conditions, and secondary soils. It is therefore found in a wide variety of habitats: volcanic terrains, lava-strewn coasts, and clearings or limestone outcrops. It can grow up to 9 metres (30 ft) tall, and has large, simple, dark green, shiny and deeply veined leaves. The plant flowers and fruits all year round and produces a small white flower. The fruit is a multiple fruit that has a pungent odor when ripening, and is hence also known as cheese fruit or even vomit fruit. It is oval and reaches 47 centimetres (1.62.8 in) in size. At first green, the fruit turns yellow then almost white as it ripens. It contains many seeds. It is sometimes called starvation fruit. Despite its strong smell and bitter taste, the fruit is nevertheless eaten as a famine food and, in some Pacific islands, even a staple food, either raw or cooked. Southeast Asians and Australian Aborigines consume the fruit raw with salt or cook it with curry. The seeds are edible when roasted. The noni is especially attractive to weaver ants, which make nests out of the leaves of the tree. These ants protect the plant from some plant-parasitic insects. The smell of the fruit also attracts fruit bats, which aid in dispersing the seeds. Noni fruit ...
  • The Culture of Primary Motor and Sensory Neurons in Defined Media Watch video protocols on JoVE - . Journal of Visualized Experiments (JoVE) is a scientific journal publishing video articles on biological experiments filmed at leading research institutions. JoVE is the first video journal indexed in PubMed. To see more of this protocol go to The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds This video protocol was filmed at the University of Michigan. Electrospinning is a technique for producing micro- to nano-scale fibers. Fibers can be electrospun with varying degrees of alignment, from highly aligned to completely random. In addition, fibers can be spun from a variety of materials, including biodegradable polymers such as poly-L-lactic acid (PLLA). These characteristics make electrospun fibers suitable for a variety of scaffolding applications in tissue engineering. Our focus is on the use of aligned electrospun fibers for nerve regeneration. We have previously shown that aligned electrospun PLLA fibers direct the outgrowth of both primary sensory and motor neurons in vitro. We maintain that the use of a primary cell culture system is essential when evaluating biomaterials to model real neurons found in vivo as closely as possible. Here, we describe techniques used in our laboratory to electrospin fibrous scaffolds and culture dorsal root ganglia explants, as well as dissociated sensory and motor neurons, on electrospun scaffolds. However, the ...
  • Gordy's Tissue Culture Lab Gordon takes explants for his tissue culture lab. Pansy, begonia, and wandering jew.
  • biosights: May 14, 2012 - A new way forward for fibroblasts Fibroblasts moving across two-dimensional surfaces form lamellipodial protrusions at their leading edge, but how cells move through three-dimensional environments is less well understood. Petrie et al. reveal that, depending on the strength of intracellular RhoA signaling and on the elastic properties of the extracellular matrix, fibroblasts can migrate through 3D environments using either lamellipodia or blunt, cylindrical protrusions called lobopodia. This biosights episode presents the paper by Petrie et al. from the April 30, 2012, issue of The Journal of Cell Biology and includes an interview with first author Ryan Petrie (NIH, Bethesda, MD). Produced by Caitlin Sedwick and Ben Short. See the associated paper in JCB for details on the funding provided to support this original research:
  • Choice of explant The tissue obtained from the plant to culture is called an explant. The choice of explant material also determines if the plantlets developed via tissue culture are haploid or diploid
  • Carrot Transformation with Agrobacterium: University of Leicester practical class BS1003 This videoblog from is about one of the Cell and Developmental Biology practicals that I run at the University of Leicester for course #BS1003. It involves infection of carrot root slices with three strains of Agrobacterium, two of which cause the plant cells to divide. An earlier video showed how we set up the cultures /watch?v=mS6OjCekrNo; here we review the results and look at the Agrobacterium plasmid structure. Plant development can be disrupted dramatically by certain pathogens. Here, we saw how the bacterial pathogen, Agrobacterium tumefaciens, causes tumours on differentiated plant tissues by activating cell division. The process involves the transfer of bacterial genes into the plant chromosomes at wound sites, resulting in the genetic transformation of the plant cells which then divide in an uncontrolled fashion because they have incorporated the genes for hormone production into the carrot cells. These genes are located in a circular DNA molecule present in the wild type Agrobacterium tumefaciens known as T37. This is the Ti plasmid, some 206000bp long. The part that is transferred into the nuclear DNA of the carrot is known as the T-DNA and it contains the genes which make the hormones that induce the plant cells to divide. A different group of genes - both for hormones and controlling plant developement - are present in Agrobacterium rhizogenes and these lead to production of differentiated roots from the carrot cells at the ...
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  • Beating chick (chicken) embryo heart explant I dissected a chick embryo, and it was still beating the next day. This is just an explant (chunk of tissue).
  • Circumcision 1 of 2 AS YOU KNOW I AM A VERY OPEN MINDED PERSON THIS VID IS NOT TO OFFEND YOUR BELIEFS...JUST TO HELP IMPROVE THE LIVES OF OUR SONS AND DAUGHTERS ....I HOPE. If it is not a medical emergency...why do it nowadays???? if the only point in her vid was to quote words from an OLD SERVEY OR FUNCTION then some things do not apply today. the point is for me is that today it is not necessary and our sons can then make that choice for themselves. As well as our daughters...if they were ever in that situation. HERS HIS CENTER FOR DISEASE CONTROL LINK WITH ALL THE STATS IN UR FACE ON THIS ONE PAGE. ...........my only bible when it comes to stats on DISease/AIDS www.cdc.gov THANK YOU JOEL FOR THIS GREAT INFO............. 10 Reasons NOT to Circumcise Your Baby Boy The Facts Behind Circumcision FGM - circumcision comparison: Foreskin most sensitive part of the penis in British Journal of Urology: To look like dad? -------------------------------------------------------------------- What they do with discarded infant foreskins: "A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells " "tissue explants and fibroblast cultures are established from freshly harvested neonatal foreskin tissue." m.nih.gov "bioengineered from infant foreskin tissue ...
  • Five dorsal explants in a collagen gel This video was taken for the detailed account presented in Current Protocols in Neuroscience Supplement 43, 3.20.1-3.20.17
  • Explant culture skin.avi Phasecontrast timelapse of a skin explant (piece of tissue). The explant sits in the lower right hand corner, the cells are growing out radially from the phase-bright edge of the tissue onto the free substrate. There are more and more cells by movement and division at the same time. Mammalian cells can be grown over prolonged periods in tissue culture [ by repeated subculture, but how does one get the first set of cells? One possibilities is to digest the extracellular matrix away and sediment the cells liberated (ie by centrifugation). Another possibility is "explant culture" [ illustrated here: one takes a piece of tissue (here skin) chops it into very small pieces (smaller 1mm in any direction) and places the pieces into a dish or flask with a small amount of appropriate tissue culture medium (here L14, 10%FCS, antibiotics, glutamine) medium (0.5-1ml/25cm^2 flask). Cells have membrane bound matrix metalloproteases and also release such enzymes which digest away at the extracellular matrix, allowing the cells to crawl out of the piece of tissue onto the tissue culture substrate. These cells can later easily be digested away from the surface using trypsin, and can be maintained in cell culture. After a short period of repeated culture these cells will have lost crucial markers of differentiation though. Technicalities: Time lapse taken on a Zeiss Axiovert 25 using a 10x objective, a CCD camera, an Alrad imaging board and software at 1 ...
  • Received an Implant and now you Must Undergo Revision Surgery - NY Attorney Dan Burke Attorney Dan Burke of Parker Waichman talks about how to prove you're injured with a defective medical product. With any case, it's important to show how you were injured. But if you're injured with a defective medical product, how to you prove that once it's been fixed the second time around? How we approach this is to make sure you speak to lawyer before any revision surgery. If we can do this, we have the ability to talk to your doctor and the hospital in order to preserve the defective device. This will act as evidence in your case. Once the device is removed during surgery, we will have a third party put it in storage to ensure that it is not modified or changed in any way. This proper chain of custody allows us to use the defective device to prove your case. We can have our experts review the device and show how you've been injured and how the device was defective. Watch this video now to learn more. To learn more about defective medical products, I encourage you to watch the video above and to explore our educational website at If you have legal questions, I want you to call me at (800) 800-9797. I welcome your call. Dan Burke Parker | Waichman LLP 6 Harbor Park Drive Port Washington, NY 11050 (800) 800-9797
  • Y. Bogdanova's PhD Dissertation Defense (part #1) new Experimental long-term in vitro cultivation of summer snowflake (Leucojum aestivum L.) - propagation potential, alkaloid profile and clone selection Sofia, Bulgaria, June 2010 (Presentation in Bulgarian language, subtitles in English)
  • Lindsay.wmv Hi Lindsay here you are my video clip about me. and I tell you in this video why I'd like that you come to saltillo, and what we can do. . please promise me you will see all the video is kinda long but please. try!. the last 8 min are the best. You should put the subtitles. hehe because I was nervous and well just check it..
  • Heart-Lung Explant - Gross Anatomy Exhibit heart-lung en bloc to highlight structural relationships of pulmonary artery and vein
  • My Breast Implants Come Out I get my breast implants out, that's a breast explant
  • Concise Modular Calculus [42/97]: Newton's Method (3/3 on Numerical Methods) Introduces Newton's method geometrically. Solves equations with Newton's method. Derives a recursive scheme to take square roots. All videos and slides for single variable calculus available at www2.latech.edu All videos and slides for multivariable calculus available at www2.latech.edu Public domain version of the electronic calculus textbook available at www.latech.edu
  • Bras after Explant Surgery Now that you have had your surgery to remove your Breast Implants, you will need to wear the correct bra. As you know the time straight after surgery is when the healing takes place so it is crucial you do everything you can to ensure yourself a trouble free recovery.
  • Callus Initation Transforming carrot explants with green fluorescent protein
  • Living in a Microbial World: Deciphering the Molecular Language of Partnership Living in a Microbial World: Deciphering the Molecular Language of Partnership Air date: Wednesday, January 16, 2013, 3:00:00 PM Description: Wednesday Afternoon Lecture Series Humans are products of their evolutionary history, and ample evidence suggests that humans and their microbial communities have coevolved. Thus, an understanding of the patterns of animal-microbe evolution promises to provide insight into the most critical features of these relationships. For instance, the discovery of characteristics that are conserved over evolutionary time is likely to reveal the basic principles underlying the establishment and persistence of these cross-domain symbioses. This presentation will explore: 1) the trends in host-microbe interaction across the animal kingdom, 2) the evidence that such alliances have driven the evolutionary trajectory of the partners, and 3) how studying a diversity of symbiotic models can reveal common themes of how these relationships function. Author: Margaret McFall-Ngai, Ph.D., University of Wisconsin-Madison Runtime: 01:04:53 Permanent link: videocast.nih.gov
  • Ciliated Frog Lung Cells Background: In recent years, there has been a massive decline in the amphibian populations. Many attribute this to one of three concerns (pathogens, climate change, and pollution), or due the overwhelming combination of all three. The overall goal of this project is to determine the damaging effects of heavy metal pollutants on frog cells. Therefore the first step is to establish cell lines for three tissues (lung, skin, and gonad) from six different frog species, (Rana pipiens, Xenopus laevis, Xenopus tropicalis, Rana catesbeiana, Hyla cinerea, and Agalychnis callidryas), with a total of three males and three females for each species. Amphibians are considered to be an environmental marker. Frogs are a species found throughout many parts of the world. Therefore the species that were chosen to start amphibian cell line studies were picked to be from different parts of the globe as well as different areas that they live in nature. Experimental Studies: We are currently developing cell lines. Once cell lines are developed we will begin expierments. Cell lines will be developed by harvesting tissues using explants and collaganese digestion protocols, and then immortalized using telomerase. Once cell lines have been established, genotoxic and cytotoxic tests will be done using carcinogenic metals such as chromium compounds. We hope to have a working cell line by spring of 2010. References: Sparling, Donald W., Greg Linder, and Christine A. Bishop. Excotoxicoloy of Amphibians ...
  • Calcium Signaling in Neuronal Motility: Supplemental Video A supplemental video from the 2007 review by James Q. Zheng and Mu-ming Poo, "Calcium Signaling in Neuronal Motility," from the Annual Review of Cell and Developmental Biology: A time-lapse sequence of various motile behaviors of the growth cone in culture. The explant cultures were made from rat pontine nuclei and plated on laminin substrate. Many axons emerged from the explants within 24 h after plating, and each of them exhibits a motile growth cone. Different motile behaviors of the growth cones are indicated by the number labels: (1) rapid extension, (2) pausing/stalling, (3) rapid migration along other axons, (4) selective fasciculation, and (5) collapse. The playback speed is 190 times of the real time.
  • Dissection: Cut dorsal spinal cord explants Cutting 75-100um explants from a dorsal strip from an embryonic rat spinal cord. This video was taken for the detailed account presented in Current Protocols in Neuroscience Supplement 43, 3.20.1-3.20.17
  • GOP Plan To Raise Your Taxes, Cut Taxes On Rich New proposals by Republicans in Congress pretend to raise tax revenue but in fact they cut taxes on the rich and increase them on the working/middle class. The Young Turks host Cenk Uygur explains. Subscribe to The Young Turks: bit.ly The Largest Online New Show in the World. Google+: www.gplus.to Facebook: Twitter:
  • how to hack psp version 6.60 for other info contact me on gmail. additsharma205@ (first step) for 6.60 original update link :- MY BLOG (MUST CLICK... HERE ALL THINGS ARE HERE THAT U NEED) pspcfwandstuff.blogspot.in (if your system version is already in 6.60 then there is no use of downloading it) ( second step) for the hack link ( 6.60 pro b9) new link for 6.60 pro b9 www.2 (this is must to download) INSTRUCTION download both 6.60 & 6.60 pro b9 plug in your psp then go to the folder named GAME in psp (during plug in) in GAME folder make another folder named UPDATE (in capslock) put 6.60 original update in it then extract 6.60 pro b9 in the folder of GAME there would be 1CIPL FLASHER 2FASTRECOYERY 3PROUPDATE and slk in not in use so delete it then plug out your psp in psp go to game then memory stick there would be 660 PRO-B Fast Recovery then click x thats it there is your 6.60 pro b9 delete cipl flasher just keep fast recovery and pro update in memory stick thanks (its tested on psp 1000 ,psp 2000 & psp 3000) please RATE AND SUBSCRIBE ( NO CHANCE OF BRICKING ) FOR gAMES I will MAKE ANOTHER VIDEO iN future ... THANK U
  • Did You Have to Have the Mirena IUD Removed? Attorney Gary Falkowitz of Parker Waichman Attorney Gary Falkowitz of Parker Waichman lets you know how the birth control device Mirena is causing harm to women. Unlike oral contraceptives like the "pill", Mirena is a device inserted into the uterus wall of a woman. The small device is supposed to last 5 years and is marketed as being one of the most convenient options available. Headaches, nausea, and a decreased *** drive are some of the common side effects of Mirena. However, studies are showing that Mirena is causing some serious side effects. These complications include inflammatory diseases, perforation of the cervix, migration of the product, and can actually embed into the uterine wall. With a device that is prescribed to more than 2 million people in the country, the risk for harm can be pretty high. Some women have experienced issues immediately upon insertion including depression, jaundice, or severe pain within a few hours. If you or a loved one has experienced any issues while using Mirena, seek the advice of an experienced lawyer to help you with questions you may have. Watch this video now to learn more. To learn more about prescription drug law, I encourage you to watch the video above and to explore our educational website at If you have legal questions, I want you to call me at (800) 800-9797. I welcome your call. Gary Falkowitz Parker | Waichman LLP 6 Harbor Park Drive Port Washington, NY 11050 (800) 800-9797
  • Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells Watch video protocols on JoVE - . Journal of Visualized Experiments (JoVE) is a scientific journal publishing video articles on biological experiments filmed at leading research institutions. JoVE is the first video journal indexed in PubMed. To see more of this protocol go to Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells This video protocol was filmed at the University of Birmingham. Cultured embryonic and adult skeletal muscle cells have a number of different uses. The micro-dissected explants technique described in this chapter is a robust and reliable method for isolating relatively large numbers of proliferative skeletal muscle cells from juvenile, adult or embryonic muscles as a source of skeletal muscle stem cells. The authors have used micro-dissected explant cultures to ***yse the growth characteristics of skeletal muscle cells in wild-type and dystrophic muscles. Each of the components of tissue growth, namely cell survival, proliferation, senescence and differentiation can be ***ysed separately using the methods described here. The net effect of all components of growth can be established by means of measuring explant outgrowth rates. The micro-explant method can be used to establish primary cultures from a wide range of different muscle types and ages and, as described here, has been adapted by the authors to enable the isolation of embryonic skeletal muscle precursors. Uniquely ...
  • Concise Modular Calculus [76/97]: Iterated Integrals and Fubini's Theorem (2/6 on Integration of Multivariable Functions) Introduces Fubini's Theorem as a much needed tool to avoid constant use of Riemann sums. Explains the connection between multiple integrals and iterated single integrals. All videos and slides for single variable calculus available at www2.latech.edu All videos and slides for multivariable calculus available at www2.latech.edu Public domain version of the electronic calculus textbook available at www.latech.edu
  • Advanced SEO for Dealers: What Still Works on Google Everyone who does anything with digital marketing knows that Google constantly changes and massages its algorithm to improve local results. In just the last 12 months, Google has come out with 3 major updates (Panda, Venice and Penguin). While many dealerships have been left scrambling and confused in the wake of these changes, Google's updates have also created great opportunities for those dealers with a strong and diverse online marketing strategy, since most of the changes are targeted at penalizing redundant content and rewarding highly localized, relevant content for local searchers. DealerOn's CEO, Ali Amirrezvani, will cut through the clutter about what still drives organic rankings and how to leverage that knowledge for your dealership in 2013. He will also provide dealers with a list of best practices for optimization that still work in Google's new more complex SEO landscape. This free 1 hour webinar will cover the following action items: 1. Learn to Google-ize your mobile site 2. Gain insight to dramatically increase organic traffic from in-market shoppers 3. Plan for Google's next update 4. Discover tools to track and measure results 5. Learn the future of SEO for car dealers If you want to stay on top of Google's local listings and immediately improve your SEO then this is one webinar you can't afford to miss! PRESENTER: Ali Amirrezvani is the President, CEO, and Co-Founder of DealerOn, Inc., an award winning website development company and digital agency ...
  • Midweek Politics w/David Pakman Conservative Morality in Media President Robert Peters Part 1 of 2 --Robert Peters, President of the conservative group Morality in Media, joins us live to discuss the recent ruling on FCC expletive and indecency rules, including his desire for there not to be any profanity, indecency, or blasphemy of any kind on broadcast media. Broadcast on July 21, 2010 Midweek Politics with David Pakman is a nationally syndicated talk radio and program. 24/7 Voicemail Line: (219)-2DAVIDP http
  • Taro Tissue Culture Check out www.ctahr.hawaii.edu/oc/freepubs/pdf/RES-145.pdf for info about taro tissue culturing. Two week old apical stem taro tissue culture report. This is like my fifth try at it. Previously I had problems with mold growing on my explants. But now I'm using PPM (Plant Preservative Mixture) which is like a way overpriced diluted form of Kathon. So no more fungus/mold! Pretty nice....
  • I, Internet [26C3] I, Internet We are more Borg than we thought The actions of a highly-networked group of individuals cannot be explained as the sum of actions of individuals. Let's explore how far we are along with evolving collective consciousnesses and what's on their minds. With the Internet, the individual's ability for inward and especially outward communication is unprecendented in history. Millions of individuals are sharing thoughts and observations through social networks. The faster and more efficient the information flows within such a network, the harder it becomes to distinguish these processes from the activity in a biological neural network (brain). One of many thriving examples of an Internet-based social network is Twitter. A typical Twitter neuron can reach between 50 and 200 neighboring neurons. Spread-worthy information will pass this filter layer on to their respective neighbors and so on. Thus a typical Twitter neuron can reach 2500 to 40000 other neurons within just two hops -- as long as the information is worth spreading. When ***yzing the social graph, you will find highly networked clusters of individuals that share common interests. Once an important information reaches such a cluster, the ongoing mostly internal discussion significantly rises local activity, which in turn will alert neighboring clusters -- say of CIOs and media ***ysts who will pick out the relevant bits of information and carry them on to the next clusters or even out of the network. The ...
  • Neuron contact avoidance in vitro Here's a good example of contact avoidance. Two axons approach each other growing from explants in a collagen gel. One from the lower left and another is seen from the upper right. When the lower one contacts the other, it collapses its fan-like leading edge. This process repeats itself as they continue to grow in parallel. (1 image taken every 30s).
  • Michael Gutkin (Biology) Michael Gutkin, a 2010 graduating senior at Wagner College (BS in Biology), delivers his thesis presentation on "Cellular Events in Zebrafish Optic Tectal Brain Explants: A Model to Study Nerotrauma and Neuroregeneration."