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antonospora

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  • end of the insert using M13F and M13R primers At present we use an Applied Biosystems 3730XL capillary sequencer which generates reads of over 850 high quality bases PHRED score >20 Sequences are assembled using the ARACHNE assembler and ORFs are called on stable contigs over 3000bp using Glimmer2 0 and CRITICA
  • Glimmer2 0 and CRITICA The estimated size of the Antonospora locustae genome is 5 300 000 nucleotides Our general genome sequencing strategy is to rely upon a shotgun approach to maximize recovery of

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