Part 1 of 6 Antibody based Biomarker Discovery in Cancer Research We recently showed you where to find the wealth of IHC data available from Human Protein Atlas web portal. Now you can listen to Dr. Fredrik Ponten, the Vice-program Director of Human Protein Atlas Project describe an antibody-based proteomics strategy to identify cancer biomarkers using Human Protein Atlas data. The strategy combines high-throughput generation of mono-specific antibodies with protein profiling in human tissues and cells using immunohistochemistry (IHC) on cell and tissue microarrays (TMAs). Dr. Ponten covers the development and validation of Prestige Antibodies® powered by Atlas Antibodies and explores the use of these antibodies in the search and characterization of biomarkers related to cancer. The webinar concludes with a brief overview of Your Favorite Gene Powered by Ingenuity. ( Speaker: Dr. Fredrik Ponten, Professor, Uppsala University and Vice-program Director, Human Protein Atlas Project
Antibody find spurs fresh hopes for AIDS vaccine US scientists identify antibodies that inhibit HIV. Researchers at the US National Institute of Allergy and Infectious Diseases (NIAID) at the National Institutes of Health built on a decade of research in their recent discovery of two antibodies that are naturally produced by humans, which can neutralize 90% of HIV viral strains. NIAID Director Dr. Anthony Fauci stated that the broad properties of these antibodies, which prevent most HIV strains from infecting human cells, make their discovery all the more exciting in being able to further speed efforts toward a preventive HIV vaccine for global use. Bravo and our sincere gratitude, Dr. Fauci, fellow National Institute of Allergy and Infectious Diseases scientists and all others for your research efforts that have culminated in this valued find. Wishing your project continued success in bringing benefit to millions across the world.
Review of Antibody and Cell-mediated Immunity Review of immune system
Measuring Serum Antibody with an Agglutination Assay This short animation demonstrates measuring serum antibody with an agglutination assay. This resource was developed by Cary Engleberg of the University of Michigan. It is part of a larger learning module about laboratory methods for clinical microbiology (available at open.umich.edu ? 2009-2010, Cary Engleberg. This is licensed under a Creative Commons Attribution Noncommercial 3.0 License
Antibody Purification (Protein A Column) ( ) - Protein A Sepharose is prepared by covalently coupling Protein A to 6% cross-linked sepharose beads. The coupling technique is optimized to give a high binding capacity for IgG. This protocol is a simple, reliable method for purifying total IgG from crude protein mixtures such as serum or ascites fluid. More videos at Abnova http
Fumiya Tanaka - "Antibody" TRMLP001, 1997
New developed antibody - 3D Animation Hybrid Medical Animation CD28 and CTLA-4, together with their ligands, B7-1 and B7-2, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA-4 is up-regulated rapidly following T cell activation and CD28 ligation. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28. Watch more 3D animations in my channel.
3D Medical Animation: Antibody Immune Response This 3D medical animation shows how antibodies stop harmful pathogens from attaching themselves to healthy cells in the blood stream. The animation begins by showing normal red and white blood cells flowing through the blood stream. Next, a single pathogen appears onscreen slowly moving toward its destination on the surface of a cell. The tubular extensions on the pathogen are surface proteins which attach to corresponding surface proteins on a white blood cell, or leukocyte. As the animation continues, more pathogens continue to attach to the white blood cell, rendering it ineffective. During the immune system response, Y-shaped antibodies begin attacking the pathogen, binding to its surface proteins as the pathogen attempts to anchor to the blood cell. The antibodies completely block the pathogen from attaching to the blood cell, "tagging" the pathogen so that one of the immune system's leaner cells, a macrophage, appears onscreen to engulf and digest the pathogen. Nucleus Medical Art is a leading creator and licensor of medical illustrations, 3D medical animations and interactive multimedia for medical devices, pharmaceutical companies, education, biotechnology, advertising agencies, lawyers, and more. Online at
HIV/AIDS cure - Antibody That Kills 91% of HIV Strains Antibody Discovered for 91% of HIV Strains
The Antibody Song We recorded all of the music on garage band and edited the movie using imovie. The whole video was filmed at Stanford University. Music performed and recorded by Devin King. Lyrics performed and written by Lnzz Liebson. Music video edited by Lnzz and Devin. The song is based off of Jason Mraz's "Curbside Prophet." Lyrics: CHORUS Im just an antibody floatin round in your body waiting for a foreign antigen. Im just an antibody floatin round in your body waiting for an antigen to come in. Im just an antibody floatin round in your body waiting for a foreign antigen. Im just an antibody floatin round in your body waiting for an antigen to come in. Yo Yo it all started way back in the bone marrow A new B cell was made and through the marrow it flowed Bumping into self-antigens down the road But it didnt bind them instead it let them go I was accepted as an antibody wasnt rejected self-antigens I inspected, but always neglected well Look at me now, Look at me now, Look at me now now now now CHORUS Well then youre never going to guess What Ive seen seen seen Cause I have no address and I spend my days mess- Ing around and floating down around in your blood stream Well you know that Ive been looking out for Something to bind to my variable region Looking for antigens that will stick with me instead of leaving A foreign antigen I look forward to receiving Im on a one-man mission as I go interweaving With my eye on the prize suddenly hypnotized Because I bind something strange now ...
Leslie Kendall talks about Body/Antibody on Kansas City Live Lead actress Leslie Kendall of the film BODY/ANTIBODY discusses the movie on "Kansas City Live" before its screening at the Kansas City Int'l Film Festival. BODY/ANTIBODY is directed by Kerry Douglas Dye and Jordan Hoffman, and stars Robert Gomes, Leslie Kendall, Frank Deal, and Deborah Gibson. More info at .
Body/Antibody Trailer Body/Antibody is LeisureSuit Media's 2007 release. It stars Robert Gomes, Leslie Kendall, Frank Deal, and Deborah Gibson, and was Directed by Kerry Douglas Dye and Jordan Hoffman.
HIV Test - A Global Fraud Tomás Brewster's journey in a brief 9 minute excerpt about the false "hiv" positive mis-diagnosis he received 6 years after he took the deadly HIV drugs Indinivir, AZT ,3TC, & also Combivir and Nevirapine(Viramune). Testing Antibody Positive in 1997 he was later told in 2004 that he was "Indeterminate" and needed to re-test for HIV or be let go from funding and medical care, period. Not making any sense of any of this, the HIV paradigm has sent him through the vast questions of the HIV Industry and the HIV/AIDS Dissident movement. Technically HIV Positive for 13 years he has banned the Toxic drug regimens for the last 7 years, and is alive and very healthy inspite of an 8 year prognosis of death and the contrarian phony "AIDS" label that he was told he'd get. Tomás has moved forward only to share his story with people and to warn other people about the inaccurate & non-specific HIV Testing campaigns and the deadly life ending HIV Drug Treatments that actually cause a multitude of Toxic Induced Diseases that supercede the common AMERICAN definition for "AIDS" and physical body deformities. ++++++++++++++++++++++++++++++++ Well documented "Factors Known to cause false positive HIV Tests .za But False-Positive for What? +++++++++++++++++++++++++++++++++ Nevirapin/ Viramune www.accessdata.fda.gov ++++++++++++++++++++++++++++++++++++++++ Combivir www.accessdata.fda.gov +++++++++++++++++++++++++++++++++++++++++++ Indinivir/Crixivan www.accessdata.fda.gov ++++++++++++ ...
Antibody Conjugation - FITC ( ) - The conjugation of polyclonal and monoclonal antibodies with fluorescein isothiocyanate (FITC) is used in immunohistochemistry and immunofluorescence studies. FITC isomer I is a widely used fluorescent labeling reagents due to the fluorophore's high quantum efficiency and conjugate stability. More videos at Abnova http
Antibodies "Antibodies of the immunoglobulin G class are Y-shaped glycoproteins that circulate in the bloodstream. They bind to and inactivate foreign molecules—the antigens—and mark them for destruction. Each IgG molecule consists of two light chains and two heavy chains. The heavy chains have carbohydrates attached. The regions of the antibody that bind to antigens are located at the very tips of the two arms. Each arm of the antibody is composed of four domains. Two are called the variable domains, contributed by the heavy and light chains, and hence called VH and VL. The variable domains are attached to two constant domains, again one each from the heavy and light chains, and hence called CH and CL. Variable and constant domains share a similar structure, called the Ig fold. Each domain consists of a pair of beta sheets, one with three strands and one with five. A single covalent disulfide bridge holds the two sheets together, which results in a rigid and very stable domain. As their name implies, the variable domains vary in amino acid sequence from one antibody molecule to another, thus providing the vast diversity in structure required by the immune system. The antigen-binding site in the variable domains is composed of hypervariable loops that are especially susceptible to sequence variations. Sequence variations in the hypervariable loops are responsible for the specificity of antibodies to particular antigens. Antigens bind to the tip of each antibody arm, generally two ...
Specific Immunity, Antibodies
Home Work #1 Antibody Movie This movie depicts one of the classically Y-shaped antibody molecules. Specifically, this is a rotating Immunoglobulin G, IgG (PDB ID 1IGT), which is the dominant class of antibodies found within the body and also the first to be structurally ***yzed through X-Ray Crystallography. The holes found within the upper Y branches are the sites where the antibody binds the antigen substrate and are known as the Fab regions for antigen-binding fragments. The lower Y branch is called the Fc region for crystallizes readily. These holes have a 3-d structure, charge density, ability to H-bond, and hydrophobic regions that is created by the unique pairing of a heavy chain (blue) with a light chain (yellow), which is determined by the amino acid residues found in that particular antibody. The heavy and light chains are linked by covalent and disulfide bonds. These pockets can only recognize a particular molecular structure found on the antigen. It is this high degree of a lock and key fit that enables the great specificity by which antibodies bind a type of antigen. Upon binding an antigen, antibodies undergo a conformational change that serves to tighten its grip upon the antigen and this is the reason why the Kd is so low at ~10^-10 M. Sean Ponce
Can you smell me ... I'm your antibody (Eisbrecher - "Antikoerper" Chris Keller version) One of my favorite Eisbrecher-songs; for the English lyrics watch out this: Can you smell me Can you feel me I have come to seduce you I creep up slowly from behind so I can devour you completely Every part of you is familiar to me I feel good so good in your skin I am your antibody I am the parasite Antibody that loves your body I am your antibody I am your legionnaire Antibody Let me defend you I am your antibody I am the parasite Antibody that loves your body I am your antibody that conquers all enemies Antibody that drowns in your blood Can you smell me Can you hear me I was made to captivate you It is so nice with you so totally alone and I penetrate deeper ever deeper into you Every part of you is my home I will stay true to you and never move back out I am your antibody I am the parasite Antibody that loves your body I am your antibody I am your legionnaire Antibody Not a cowardly deserter no (Translation ©2006 Jeremy Williams; taken from www.eis-) copyrights held by others and all the artists are not involved with this vid. It's just a fanvid and not an official one, please watch my interpretation of this song and enjoy it! Attention: this vid contains adult/homo***ual/*** themes!
"Immune" Documentary - Part 6 - B Cells & Antibody Release Part 6 of my documentary for Biology class.
Optical Antigen-Antibody Reaction Measurement Instrument DigInfo - movie.diginfo.tv Takuo Akimoto's research group at the Tokyo University of Technology is looking for a partner to commercialize a portable optical antigen-antibody reaction measurement instrument the group developed. The instrument uses surface plasmon resonance, a phenomenon in which the intensify of reflected light attenuates as the laser incidence angle changes, to measure antigen-antibody reactions simply. Antibodies are affixed to the tip of a glass sensor probe, which is 1.5mm in diameter and 50mm in length, and the probe tip is submerged in a measurement sample. The sample can be as little as 10 microliters. The instrument is 20cm long and 10cm wide, small enough to be carried around. The research group plans to make the instrument smaller and even more accurate and develop more applications for commercializing it.
HIV Vaccine Design and the Neutralizing Antibody Problem In this series from the CFAR National Symposium on HIV/AIDS Prevention & Transmission 2007, you will hear from experts from universities throughout the US and from South Africa, updating us on their latest research and findings. Join Dennis Burton, PhD, The Scripps Research Institute, as he presents on HIV Vaccine Design and the Neutralizing Antibody Problem. Series: "CFAR, UCSD Center for AIDS Research " [3/2008] [Health and Medicine] [Show ID: 13710]
Antibody Game! Save the world by healing an infection! Play the role of a young scientist who is trying to save the world by fighting an infection that threatens mankind with extinction. Spread antibodies that would take over the cells and will eventually remove the contagion. Become the dominant species and heal the plague through 50 levels of increasing difficulty. Antibody game is designed for android phones starting with the 1.5 version of the OS and the screen resolution of 320x480. The full and lite versions of the game are available on the Android Market. Game site -
Antibody Mediated Immune Response
Eisbrecher - Antikörper The song Antikörper (Antibody) by the german industrial metal band Eisbrecher, from their second album Antikörper (2006) TRANSLATIONS Can you smell me Can you feel me I have come to seduce you I creep up slowly from behind so I can devour you completely Every part of you is familiar to me I feel good so good in your skin I am your antibody I am the parasite Antibody that loves your body I am your antibody I am your legionnaire Antibody Let me defend you I am your antibody I am the parasite Antibody that loves your body I am your antibody that conquers all enemies Antibody that drowns in your blood Can you smell me Can you hear me I was made to captivate you It is so nice with you so totally alone and I penetrate deeper ever deeper into you Every part of you is my home I will stay true to you and never move back out I am your antibody I am the parasite Antibody that loves your body I am your antibody I am your legionnaire Antibody Not a cowardly deserter no
Evidence that HIV antibody tests are not specific Using the time of disappearance of maternal antibodies the Perth Group presents evidence that the HIV antibody tests are not specific for HIV infection.
Antibody Purification (Affinity) ( ) - Cyanogen bromide (CNBr) is the most common method for preparing affinity chromatography to purify antibody because of its simplicity and mild pH conditions. CNBr reacts with the hydroxyl groups on agarose to form cyanate esters and imidocarbonates. These groups are reacted with primary amines in order to couple the protein onto the agarose matrix. More videos at Abnova http
Antibody part ID ©2009 Demonstratives, Inc. A demo video I made for work, showing the different regions of an antibody ribbon structure and its structural relation to the antigen. The little lightning bolts represent binding forces (hydrogen bonds, Van Der Waals forces, etc.
Unit 6 - Animatic showing the making of an Antibody
Antibody structure - LiaGen Antibodies (also known as immunoglobulins, abbreviated Ig) are gamma globulin proteins that are found in blood or other bodily fluids of vertebrates, and are used by the immune system to identify and neutralize foreign objects, such as bacteria and viruses. They are typically made of basic structural units - each with two large heavy chains and two small light chains - to form, for example, monomers with one unit, dimers with two units or pentamers with five units. X-ray crystallography is a method of determining the arrangement of atoms within a crystal, in which a beam of X-rays strikes a crystal and scatters into many different directions. From the angles and intensities of these scattered beams, a crystallographer can produce a three-dimensional picture of the density of electrons within the crystal. From this electron density, the mean positions of the atoms in the crystal can be determined, as well as their chemical bonds, their disorder and sundry other information. Design & production: Kosi Gramatikoff, PhD
ASCO GI: Antibody Slows Metastatic Colon Cancer ORLANDO -- Patients with nonmutated colorectal tumors had significant improvement in progression-free survival (PFS) when the monoclonal antibody panitumumab (Vectibix) was added to conventional chemotherapy, data from two randomized clinical trials showed. When used in first-line therapy for metastatic cancer, the antibody-chemotherapy combination was associated with a 20% improvement in the hazard ratio for progression compared with chemotherapy alone. In the second-line metastatic setting, the combination improved the hazard ratio by 27%.
Antibody Trailer Technology is the way of the future and terrorists have come up with a wonderful new method - hiding the trigger for a bomb inside their bodies so if they are killed, the bomb goes off. Henriksen, an explosives expert and a team of specially trained scientists are sent to deactivate a trigger for a nuclear weapon that will vaporize Munich and contaminate Western Europe, the trigger is of course inside a loopy terrorist's body. To get it, the team are shrunk down and injected into the terrorist. Then begins a fantastic voyage as they search for the microchip detonator. The scientists take an internal journey through the wonders of the human body, but also must fight off a constant attack from anti-bodies, white corpuscles and viruses. Walking in another man's footsteps is one thing; to swim in his blood is something else.
Blood Testing Facts : What is an Antibody in Blood? Testing for antibodies is important in blood screening. Learn how it works in this free video clip about the facts of blood donation. Expert: Claudia Benekie Bio: Dr. Claudia Benekie is a research laboratory director of Stanford Blood Center. Filmmaker: Bing Hu
MidiMiliz - Antibody Antibody
Part 4 of 6 Antibody based Biomarker Discovery in Cancer Research We recently showed you where to find the wealth of IHC data available from Human Protein Atlas web portal. Now you can listen to Dr. Fredrik Ponten, the Vice-program Director of Human Protein Atlas Project describe an antibody-based proteomics strategy to identify cancer biomarkers using Human Protein Atlas data. The strategy combines high-throughput generation of mono-specific antibodies with protein profiling in human tissues and cells using immunohistochemistry (IHC) on cell and tissue microarrays (TMAs). Dr. Ponten covers the development and validation of Prestige Antibodies® powered by Atlas Antibodies and explores the use of these antibodies in the search and characterization of biomarkers related to cancer. The webinar concludes with a brief overview of Your Favorite Gene Powered by Ingenuity. ( Speaker: Dr. Fredrik Ponten, Professor, Uppsala University and Vice-program Director, Human Protein Atlas Project
Say, Antibody! (Can You Feel The Sunshine) After seeing many songs being played backwards, I decided to do one of my own. I hope you enjoy! Edit: I'm through. Any further discussion of Tails Doll garbage WILL GET YOU MUTED.
Antibody Pair for Protein Protein Interaction and Protein Phosphorylation ( ) - A powerful platform for protein-protein interaction and protein phosphorylation studies in situ is created by combining Abnovas dual recognition, primary antibody pair with proximity ligation assay from Olink Bioscience. This revolutionary combination enables unprecedented specificity and sensitivity of protein detection and quantification for high performance immunofluorescence and immunohistochemistry applications. More videos at Abnova http
How do you make an antibody that works? SDIX Better Antigens. Better Antibodies. Better Assays. (800) 544-8881 info@ To make a successful protein antibody, SDIX considers how the antibody will work in your assay, followed by antigen strategy, immunization strategy, screening and purification. Our scientists work with you, using our uncommon competency to understand your needs to develop a quality antibody to work in your research.
Attacking influenza: how human monoclonal antibodies neutralize the flu virus This animation illustrates how directing monoclonal antibodies to the influenza virus prevents a conformational change in the protein that is necessary for viral entry into the host cell. Read more: www.dana- and see a video of Dr. Wayne Marasco of Dana-Farber Cancer Institute discussing this discovery.
Know the Facts About Antiphospholipid Antibody Syndrome (APS) Know the Facts, Signs & Symptoms of Antiphospholipid Antibody Syndrome (APS). June is Antiphospholipid Antibody Syndrome (APS) Awareness month. APS is associated with recurrent clotting events (thrombosis) including premature stroke, repeated miscarriages, phlebitis, venous thrombosis (clot in the vein) and pulmonary thromboembolism (blockage of an artery found in the lung due to a clot that has traveled from a vein). It is also associated with low platelet or blood elements that prevent bleeding. Recently, however, even more disease states have been linked with APL including premature heart attack, migraine headaches, various cardiac valvular abnormalities, skin lesions, abnormal movement/chorea, diseases that mimic multiple sclerosis, vascular diseases of the eye that can lead to visual loss and blindness. Founded in 2005, the APS Foundation of America, Inc. is the leading United States nonprofit health agency dedicated to bringing national awareness to Antiphospholipid Antibody Syndrome (APS), the major cause of multiple miscarriages, thrombosis, young strokes and heart attacks. We are a volunteer run, community based 501(c)3 non-profit Public Charity organization and is dedicated to fostering and facilitating joint efforts in the areas of education, support, public awareness, research and patient services. Our URL is Keywords APSFA, APS Foundation, antiphospholipid antibody syndrome, lupus, stroke, dvt, pe, thrombosis, clot, migraine, hughes syndrome ...
Antiphospholipid Antibody Syndrome This video was produced by the APS Foundation of America, Inc. The APSFA can be located at . APS is associated with recurrent clotting events (thrombosis) including premature stroke, repeated miscarriages, phlebitis, venous thrombosis (clot in the vein) and pulmonary thromboembolism (blockage of an artery found in the lung due to a clot that has traveled from a vein). It is also associated with low platelet or blood elements that prevent bleeding. Recently, however, even more disease states have been linked with APL including premature heart attack, migraine headaches, various cardiac valvular abnormalities, skin lesions, abnormal movement diseases that mimic multiple sclerosis, vascular diseases of the eye that can lead to visual loss and blindness. Founded in 2005, the APS Foundation of America, Inc. is the leading United States nonprofit health agency dedicated to bringing national awareness to Antiphospholipid Antibody Syndrome (APS), the major cause of multiple miscarriages, thrombosis, young strokes and heart attacks. We are a volunteer run, community based 501(c)3 non-profit Public Charity organization and is dedicated to fostering and facilitating joint efforts in the areas of education, support, public awareness, research and patient services. Their URL is The APS Friends and Support Forum at http . This is an open forum for people who have Antiphospholipid Antibody Syndrome, friends, family, and caregivers. There are many ways you can ...
Re: I have Antiphospholipid antibody syndrome... Közvetlen feltöltés videokameráról
Antiphospholipid Antibody Syndrome: A Patient's Journey Rebecca, 39, suffered a massive stroke in her mid-30s that left her almost entirely incapacitated, although she recovered under the care of her doctors. The stroke was caused by a previously undiagnosed autoimmune disorder called antiphospholipid antibody syndrome (APS), which causes blood to clot more easily and can result in early miscarriages. Now that Rebecca is pregnant with her first child, her team of doctors must closely monitor her condition to ensure a safe delivery. To learn more about APS and other blood disorders, visit ASH's patient resources webpage This clip was excerpted from the documentary film "Blood Detectives." To learn more about the film, visit